| Catalog No. | Size | Price (USD) |
| 40592-R117-20 | 20 µg | $296.22 |
| 40592-R117-100 | 100 µg | $592.44 |
General Information
| Product name | SARS-CoV-2 (2019-nCoV) Spike Neutralizing Antibody, Rabbit Mab |
| Validated applications | ELISA (FAQ Protocol), FCM (FAQ Protocol), Neutralization |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | SARS-CoV-2 Spike RBD Protein (Can block the binding between RBD and ACE2) Has cross-reactivity in ELISA with SARS-CoV-2 Delta (B.1.617.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H10) SARS-CoV-2 Delta (B.1.617.2) Spike S1+S2 Protein (Cat# 40589-V08B16) SARS-CoV-2 Delta (B.1.617.2) Spike RBD Protein (Cat# 40592-V08H90) SARS-CoV-2 Spike RBD (N501Y) Protein (Cat# 40592-V08H82) SARS-CoV-2 Spike S1+S2 ECD Protein (Catalog#40589-V08B1) SARS-CoV-2 Spike RBD Protein (Catalog#40592-V08B) Medium cross-reactivity in ELISA with SARS-CoV-2 Delta (B.1.617.2) Spike S1 Protein (Cat# 40591-V08H23) No cross-reactivity in ELISA with SARS-CoV-2 Omicron (XBB.1.16) Spike S1+S2 trimer Protein (Cat# 40589-V08H48) SARS-CoV-2 Omicron (XBB.1.5) Spike RBD Protein (Cat# 40592-V08H146) SARS-CoV-2 Omicron (XBB.1.5) Spike S1+S2 trimer Protein (Cat# 40589-V08H45) SARS-CoV-2 Omicron (XBB.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H42) SARS-CoV-2 Omicron (XBB) Spike RBD Protein (Cat# 40592-V08H144) SARS-CoV-2 Omicron (BQ.1.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H41) SARS-CoV-2 Omicron (BQ.1.1) Spike RBD Protein (Cat# 40592-V08H143) SARS-CoV-2 Omicron (BF.7) Spike S1+S2 trimer Protein (Cat# 40589-V08H39) SARS-CoV-2 Omicron (BA.4.6/BF.7) Spike RBD Protein (Cat# 40592-V08H140) SARS-CoV-2 Omicron (BA.4.6) Spike S1+S2 trimer Protein (Cat# 40589-V08H37) SARS-CoV-2 Omicron (BA.2.75) Spike RBD Protein (Cat# 40592-V08H135) SARS-CoV-2 Omicron (BA.2.75) Spike S1+S2 trimer Protein (Cat# 40589-V08H36) SARS-CoV-2 Omicron (BA.2.75.2) Spike RBD Protein (Cat# 40592-V08H141) SARS-CoV-2 Omicron (BA.2.75.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H38) SARS-CoV-2 Omicron (BA.2.3.20) Spike RBD Protein (Cat# 40592-V08H142) SARS-CoV-2 Omicron (BA.1.1) Spike S1+S2 trimer Protein (Cat# 40589-V08H29) SARS-CoV-2 Omicron (BA.1.1) Spike RBD Protein (Cat# 40592-V08H129) SARS-CoV-2 Omicron (B.1.1.529) S1+S2 trimer Protein (Cat# 40589-V08H26) SARS-CoV-2 Omicron (B.1.1.529) Spike S1 Protein (Cat# 40591-V08H41) SARS-CoV-2 Omicron (B.1.1.529) Spike RBD Protein (Cat# 40592-V08H121) SARS-CoV-2 Omicron (BA.2) Spike S1+S2 trimer Protein (Cat# 40589-V08H28) SARS-CoV-2 Omicron (BA.2) Spike S1 Protein (Cat# 40591-V08H43) SARS-CoV-2 Omicron (BA.2) Spike RBD Protein (Cat# 40592-V08H123) SARS-CoV-2 Omicron (BA.2) Spike S1 NTD Protein (Cat# 40591-V08H44) SARS-CoV-2 Omicron (BA.2.12.1) Spike RBD Protein (Cat# 40592-V08H132) SARS-CoV-2 Omicron (BA.4/BA.5/BA.5.2) Spike RBD Protein (Cat# 40592-V08H130) SARS-CoV-2 Omicron (BA.5) Spike RBD Protein (Cat# 40592-V08H131) SARS-CoV-2 XD (BA.1 x AY.4) Spike S1+S2 trimer Protein (Cat# 40589-V08H30) SARS-CoV-2 Delta (B.1.617.2) Spike S1 NTD Protein (Cat# 40591-V08H36) SARS-CoV-2 Spike RBD (K417T, E484K, N501Y) Protein (Cat# 40592-V08H86) SARS-CoV-2 Spike RBD (K417N, E484K, N501Y) Protein (Cat# 40592-V08H85) SARS-CoV (Isolate Tor2) Spike S1+S2 ECD Protein (Cat# 40634-V08B) MERS-CoV Spike S1+S2 ECD Protein (Cat# 40069-V08B) HCoV-NL63 Spike S1+S2 ECD Protein (Catalog# 40604-V08B) HCoV-229E Spike S1+S2 ECD Protein (Catalog# 40605-V08B) HCoV-HKU1 (isolate N5) Spike S1+S2 ECD Protein (Cat# 40606-V08B) HCoV-OC43 Spike S1+S2 ECD Protein (Cat# 40607-V08B) |
| Immunogen | Recombinant SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Protein (Catalog#40592-V05H) |
| Preparation | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| Source | Monoclonal Rabbit IgG |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80, pH6.0 |
| Conjugate | Unconjugated |
| Endotoxin | < 3 EU/mg |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40592-R117) was detected by SARS-CoV-2 (2019-nCoV) Inhibitor Screening ELISA Kit (Catalog# KIT001). The IC 50 (WT) is typically 0.5-5 nM. |
| Image 1 Alt Text | SARS-CoV-2 Coronavirus spike Enzyme linked immunosorbent assay (ELISA) 31959 |
| Image 1 URL | Source image |
| Image 2 Description | Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40592-R117) was detected by SARS-CoV-2 (2019-nCoV) Inhibitor Screening ELISA Kit (Catalog# KIT001). The IC 50 (WT) is typically 0.5-5 nM. |
| Image 2 Alt Text | SARS-CoV-2 Coronavirus spike Microneutralizaiton (MN) 31961 |
| Image 2 URL | Source image |
| Image 3 Description | Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody (Catalog# 40592-R117) was detected by SARS-CoV-2 (2019-nCoV) Inhibitor Screening ELISA Kit (Catalog# KIT001). The IC 50 (WT) is typically 0.5-5 nM. |
| Image 3 Alt Text | SARS-CoV-2 Coronavirus spike Flow Cytometry (FC) 32519 |
| Image 3 URL | Source image |
Background Information
| Description | The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2 (angiotensin-converting enzyme 2), DPP4 (dipeptidyl peptidase-4), APN (aminopeptidase N), CEACAM (carcinoembryonic antigen-related cell adhesion molecule), Sia (sialic acid), O-ac Sia (O-acetylated sialic acid). The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate. |
| References |
|
title: Recombinant severe respiratory syndrome coronavirus 2 Rbd trimer protein vaccine capable of generating broad-spectrum cross neutralization activity, and preparation method and use thereof
authors: Qiming Li; Yu Liang; ...; Ning Liu
date: 2025-06-10
cited_by_count: 0
techniques: Activity Assay; Binding Assay; Bioprocessing; Concentration Assay; Enzyme-linked Immunosorbent Assay; Expressing; Incubation; Mutagenesis; Negative Control; Purification; Recombinant; Sequencing; Virus
bioz_score: 93
title: Naked-Eye Readable Microarray for Rapid Profiling of Antibodies against Multiple SARS-CoV-2 Variants
authors: Jingjie Nan; Yuan Chen; ...; Bai Yang
journal: Nano Letters
date: 2023-12-04
pmid: pm38047611
cited_by_count: 0
bioz_score: 91
title: Kaempferol inhibits SARS-CoV-2 invasion by impairing heptad repeats-mediated viral fusion.
authors: Junwei Gao; Can Cao; ...; Ning Li
journal: Phytomedicine : international journal of phytotherapy and phytopharmacology
date: 2023-08-07
pmid: 37421767
doi: 10.1016/j.phymed.2023.154942
cited_by_count: 19
techniques: Positive Control; Purification
bioz_score: 91
title: COVID-19 Diagnosis and SARS-CoV-2 Strain Identification by a Rapid, Multiplexed, Point-of-Care Antibody Microarray
authors: Jacob T. Heggestad; Rhett J. Britton; ...; Ashutosh Chilkoti
journal: Analytical Chemistry
date: 2023-03-24
pmid: 36961989
doi: 10.1021/acs.analchem.2c05180
pmcid: 10068875
cited_by_count: 4
techniques: Comparison
bioz_score: 91
title: Utilization of Receptor-Binding Domain of SARS-CoV-2 Spike Protein Expressed in Escherichia coli for the Development of Neutralizing Antibody Assay
authors: Termsak Tantiwiwat; Apisitt Thaiprayoon; ...; Dujduan Waraho-Zhmayev
journal: Molecular biotechnology
date: 2022-09-14
pmid: 36103078
doi: 10.1007/s12033-022-00563-4
pmcid: 9472194
cited_by_count: 4
techniques: Variant Assay
bioz_score: 91
title: Design of a mutation-integrated trimeric RBD with broad protection against SARS-CoV-2
authors: Yu Liang; Jing Zhang; ...; Qi Ming Li
journal: Cell discovery
date: 2022-02-15
pmid: 35169113
doi: 10.1038/s41421-022-00383-5
pmcid: 8847466
cited_by_count: 24
techniques: Binding Assay; Enzyme-linked Immunosorbent Assay; Recombinant
bioz_score: 91
title: Structure and computation-guided design of a mutation-integrated trimeric RBD candidate vaccine with broad neutralization against SARS-CoV-2
authors: Liang Yu; Zhang Jing; ...; Li Qi Ming
journal: bioRxiv
date: 2021-06-18
doi: 10.1101/2021.06.18.448958
cited_by_count: 0
techniques: Activity Assay; Binding Assay; Enzyme-linked Immunosorbent Assay; Recombinant
bioz_score: 91
