| Catalog No. | Size | Price (USD) |
| 202710-T36-50 | 50 µL | $147.96 |
| 202710-T36-100 | 100 µL | $251.64 |
| 202710-T36-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-DPH4/DNAJC24 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human DPH4/DNAJC24 |
| Immunogen | E. coli-derived Human DPH4/DNAJC24 fragment |
| Preparation | Produced in rabbits immunized with E. coli-derived Human DPH4/DNAJC24 fragment, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-DPH4 Antibody; Anti-JJJ3 Antibody; Anti-ZCSL3 Antibody
Image / Experimental Information
| Image 1 Description | Anti-DNAJC24 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:20 kDa |
| Image 1 Alt Text | Human DPH4 Western blot (WB) 23637 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-DNAJC24 rabbit polyclonal antibody at 1:500 dilution Lane A: U-251 MG Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:17 kDa Observed band size:20 kDa |
| Image 2 Alt Text | Human DPH4 Immunoprecipitation(IP) 23638 |
| Image 2 URL | Source image |
Background Information
| Description | Diphthamide is a post-translationally modified histidine residue found in eukaryotic and archaeal translation elongation factor 2 (EF2). It is named after the fact that it is the target of diphtheria toxin (DT) produced by Corynebacterium diphtheriae. DT and the Pseudomonas aeruginosa exotoxin A (ETA) catalyze the ADP-ribosylation reaction on the diphthamide residue of EF2 using nicotinamide adenine dinucleotide (NAD) as the ADP-ribosyl donor. EF2 catalyzes the translocation of mRNA and peptidyl-tRNA from ribosome A site to P site. The ADP-ribosylation of EF2 inactivates it, stopping protein synthesis and leading to cell death. This modification is conserved from archaebacteria to humans. DPH4 (also known as DNAJC24, JJJ3 and ZCSL3) is 1 of several enzymes involved in synthesis of diphthamide in EEF2. |
| References |
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