| Catalog No. | Size | Price (USD) |
| 40013-H136-200 | 200 µg | $296.22 |
| 40013-H136-500 | 500 µg | $444.33 |
General Information
| Product name | Enterovirus 71 VP1 Neutralizing Antibody |
| Validated applications | Microneutralization(MN) |
| Application notes | (Antibody's applications have not been validated with corresponding viruses. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | EV Enterovirus 71 VP1 Has cross-reactivity in ELISA with EV71 genogroup C and A in cell assay No cross-reactivity in ELISA with Coxsackievirus type A16 in cell assay |
| Preparation | This antibody was obtained from human B cells by single-cell reverse transcription polymerase chain reaction (RT-PCR). |
| Source | Human IgG1 (from single cell PCR) Clone #H136 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Endotoxin | Please contact us for more information. |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | The neutralization activity of EV71 VP1 Neutralizing Antibody is Measured by Microneutralization test in vitro. The cytopathic effect of RD cells (10E5/well) induced by 100CCID50 EV71 genogroup C4 is neutralized by increasing concentrations of anti EV71 Monoclonal Antibody (Catalog 40013-H136). The IC50 is typically 0.7-1.3 µg/mL. NA: No antibody added. Validation Experiment |
| Image 1 Alt Text | EV71 Enterovirus 71 VP1 Blocking/Neutralization (B/N) 5573 |
| Image 1 URL | Source image |
title: Novel virulence determinants in VP1 regulate the assembly of enterovirus-A71.
authors: Wenjing Zhang; Quanjie Li; ...; Shan Cen
journal: Journal of virology
date: 2024-11-13
pmid: 39535185
doi: 10.1128/jvi.01655-24
cited_by_count: 1
techniques: Concentration Assay; In Vitro; Incubation; Neutralization; Transfection
bioz_score: 92
title: Inhibition of ESCRT-independent extracellular vesicles biogenesis suppresses enterovirus 71 replication and pathogenesis in mice.
authors: Yicong Liang; Yue Kong; ...; Zhen Luo
journal: International Journal of Biological Macromolecules
date: 2024-04-06
pmid: 38588842
doi: 10.1016/j.ijbiomac.2024.131453
cited_by_count: 1
techniques: Infection; Isolation; Neutralization
bioz_score: 92
title: Enterovirus A71 capsid protein VP1 increases blood–brain barrier permeability and virus receptor vimentin on the brain endothelial cells
authors: Wenjing Wang; Jiandong Sun; ...; Juan Xu
journal: Journal of neurovirology
date: 2019-09-11
pmid: 31512144
doi: 10.1007/s13365-019-00800-8
pmcid: 7040057
cited_by_count: 30
techniques: Expressing; Fluorescence; Immunofluorescence; Immunohistochemistry; In Vitro; In Vivo; Microscopy; Neutralization; Permeability; Plasmid Preparation; Quantitative RT-PCR; Staining; Transmission Assay; Western Blot
bioz_score: 92
title: The mature EV71 virion induced a broadly cross-neutralizing VP1 antibody against subtypes of the EV71 virus
authors: Chia-Ying Wu; Shu-Ling Yu; ...; Shixia Wang
journal: PLoS ONE
date: 2019-01-16
pmid: 30650163
doi: 10.1371/journal.pone.0210553
pmcid: 6334917
cited_by_count: 7
techniques: Activity Assay; Adsorption; Amplification; Binding Assay; Competitive ELISA; Enzyme-linked Immunosorbent Assay; Inhibition; Labeling; Marker; Molecular Weight; Negative Control; Neutralization; Positive Control; Produced; Purification; SDS Page; Silver Staining; Staining
bioz_score: 92
