E. coli-derived Human GSS/Glutathione Synthetase fragment
Preparation
Produced in rabbits immunized with E. coli-derived Human GSS/Glutathione Synthetase fragment, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
PBS, pH7.0 with 0.03% Proclin300
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles.
Anti-GSS/Glutathione Synthetase rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:52 kDa Observed band size:52 kDa (We are unsure as to the identity of these extra bands.)
Image 1 Alt Text
Human GSS/Glutathione Synthetase Western blot (WB) 28414
Anti-GSS/Glutathione Synthetase rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:52 kDa Observed band size:52 kDa (We are unsure as to the identity of these extra bands.)
Image 2 Alt Text
Human GSS/Glutathione Synthetase Immunohistochemistry(IHC) 28415
Anti-GSS/Glutathione Synthetase rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:52 kDa Observed band size:52 kDa (We are unsure as to the identity of these extra bands.)
Image 3 Alt Text
Human GSS/Glutathione Synthetase Immunohistochemistry(IHC) 28416
GSS (Glutathione Synthetase) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. GSS belongs to the eukaryotic GSH synthase family. Diseases associated with GSS include Glutathione Synthetase Deficiency and Glutathione Synthetase Deficiency Of Erythrocytes, Hemolytic Anemia Due To. Glutathione synthetase deficiency (GSSD) is a rare inborn error of glutathione metabolism with autosomal recessive inheritance. The severe form of the disease is characterized by acute metabolic acidosis, usually present in the neonatal period with hemolytic anemia and progressive encephalopathy.