| Catalog No. | Size | Price (USD) |
| 100028-T38-50 | 50 µL | $133.15 |
| 100028-T38-100 | 100 µL | $251.64 |
| 100028-T38-200 | 200 µL | $340.51 |
General Information
| Product name | Anti-HA tag Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ICC/IF (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: other |
| Specificity | Recognize N-terminal and C-terminal HA Tag in fusion proteins. |
| Immunogen | A synthetic peptide corresponding to the HA-tag sequence (YPYDVPDYA). |
| Preparation | Produced in rabbits immunized with a purified synthetic peptide corresponding to the HA-tag sequence. HA-tag specific IgG was purified by HA-tag affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Anti-HA Tag rabbit polyclonal antibody at 1:2000 dilution Lane A: HA-mFABP4-myc transfected 293 Cell Lysate(10ug) Lane B: HA-ARG1-myc transfected 293 Cell Lysate(10ug) Lane C: myc-mFABP4-HA transfected 293 Cell Lysate(10ug) Lane D: myc-ARG1-HA transfected 293 Cell Lysate(10ug) Lane E: Non- transfected 293 Cell lysate(10ug) Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. |
| Image 1 Alt Text | other HA tag Western blot (WB) 25858 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-HA Tag rabbit polyclonal antibody at 1:2000 dilution Lane A: HA-mFABP4-myc transfected 293 Cell Lysate(10ug) Lane B: HA-ARG1-myc transfected 293 Cell Lysate(10ug) Lane C: myc-mFABP4-HA transfected 293 Cell Lysate(10ug) Lane D: myc-ARG1-HA transfected 293 Cell Lysate(10ug) Lane E: Non- transfected 293 Cell lysate(10ug) Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. |
| Image 2 Alt Text | other HA tag Immunofluorescence(IF) 25859 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-HA Tag rabbit polyclonal antibody at 1:2000 dilution Lane A: HA-mFABP4-myc transfected 293 Cell Lysate(10ug) Lane B: HA-ARG1-myc transfected 293 Cell Lysate(10ug) Lane C: myc-mFABP4-HA transfected 293 Cell Lysate(10ug) Lane D: myc-ARG1-HA transfected 293 Cell Lysate(10ug) Lane E: Non- transfected 293 Cell lysate(10ug) Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. |
| Image 3 Alt Text | other HA tag Immunoprecipitation(IP) 25860 |
| Image 3 URL | Source image |
Background Information
| Description | The human influenza hemagglutinin (HA) epitope was derived from the protein on the HA virus. HA tag is the amino acid sequence of a YPYDVPDYA tag and a particularly common tags. HA is tagged onto proteins for study and analysis and often used in fusion protein-related research through the realization of detection of HA-tag for the detection of fusion proteins. Antibodies to HA-tag are the key of the most important tools in the detection and often used in purification of HA tag-related proteins, study of the protein interaction and detection of target protein expression. |
| References |
|
title: In Vitro Influenza A Virus-Inactivating Activity of HIDROX ® , Hydroxytyrosol-Rich Aqueous Olive Pulp Extract.
authors: Mayar Yasser Zeinelabideen Mohamed; Dulamjav Jamsransuren; ...; Yohei Takeda
journal: Pathogens (Basel, Switzerland)
date: 2025-06-25
pmid: 40559537
doi: 10.3390/pathogens14060529
cited_by_count: 0
techniques: Affinity Purification; Imaging; Membrane; Western Blot
bioz_score: 93
title: Evaporation-Enhanced Redox Cycling for Rapid Detection of Attomolar SARS-CoV-2 Virions Using Nanolithography-Free Electrochemical Devices
authors: Pouya Soltan Khamsi; Vinay Kammarchedu; Aida Ebrahimi
journal: Advanced Materials Technologies
date: 2025-05-22
cited_by_count: 0
techniques: Affinity Purification; Flow Cytometry
bioz_score: 93
title: A nisin-inducible chromosomal gene expression system based on ICE Tn 5253 of Streptococcus pneumoniae , transferable among streptococci and enterococci
authors: Mariana Tirziu; Lorenzo Colombini; ...; Francesco lannelli
journal: World Journal of Microbiology and Biotechnology
date: 2024-09-12
pmid: 39261358
pmcid: 11390789
cited_by_count: 1
techniques: Bacteria; Clone Assay; Concentration Assay; Conjugation Assay; Construct; Control; Dot Blot; Expressing; Flow Cytometry; Fluorescence; Incubation; Membrane; Plasmid Preparation; Positive Control; Purification; Quantitative RT-PCR; Recombinant; Selection; Software; Transformation Assay; Western Blot
bioz_score: 93
title: A nisin-inducible chromosomal gene expression system based on ICE Tn5253 of Streptococcus pneumoniae, transferable among streptococci and enterococci.
authors: Mariana Tirziu; Lorenzo Colombini; ...; Francesco Lannelli
journal: World Journal of Microbiology and Biotechnology
date: 2024-09-11
pmid: 39261358
doi: 10.1007/s11274-024-04124-6
cited_by_count: 1
techniques: Dot Blot; Incubation; Membrane; Purification; Software
bioz_score: 93
title: An Abies Extract Containing Nonvolatile Polyphenols Shows Virucidal Activity against SARS-CoV-2 That Is Enhanced in Increased pH Conditions.
authors: Imane Maaroufi; Dulamjav Jamsransuren; ...; Yohei Takeda
journal: Pathogens (Basel, Switzerland)
date: 2023-08-28
pmid: 37764901
doi: 10.3390/pathogens12091093
cited_by_count: 2
techniques: Acrylamide Gel Assay; Affinity Purification; Imaging; Incubation; Membrane; Nucleic Acid Electrophoresis; Western Blot
bioz_score: 93
title: Immunogenicity of COVID-eVax Delivered by Electroporation Is Moderately Impacted by Temperature and Molecular Isoforms.
authors: Federico D'Alessio; Lucia Lione; ...; Fabio Palombo
journal: Vaccines
date: 2023-03-16
pmid: 36992261
cited_by_count: 3
techniques: Affinity Purification; Incubation
bioz_score: 93
title: KIF16B mediates anterograde transport and modulates lysosomal degradation of the HIV-1 envelope glycoprotein
authors: Weaver Nicholas; Hammonds Jason; ...; Spearman Paul
journal: bioRxiv
date: 2023-03-02
doi: 10.1101/2023.03.01.530732
cited_by_count: 1
techniques: Immunofluorescence; Immunostaining; Produced; Western Blot
bioz_score: 93
title: Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
authors: Anne Harbig; Marco Mernberger; ...; Eva Böttcher-Friebertshäuser
journal: Journal of Biological Chemistry
date: 2020-08-01
doi: 10.1074/jbc.ra120.012635
cited_by_count: 30
techniques: Clone Assay; Expressing; FLAG-tag; Mutagenesis; Plasmid Preparation; Protease Inhibitor; Recombinant; Reverse Transcription Polymerase Chain Reaction; Sequencing; Synthesized
bioz_score: 93
title: Addition of αGal HyperAcute™ technology to recombinant avian influenza vaccines induces strong low-dose antibody responses
authors: Wenlan Alex Chen; Jinjin Zhang; ...; Yongchang Cao
journal: PLoS ONE
date: 2017-08-07
pmid: 28787006
doi: 10.1371/journal.pone.0182683
pmcid: 5546595
cited_by_count: 4
techniques: Blocking Assay; Electron Microscopy; Incubation; Microscopy; Modification; Negative Control; SDS Page; Staining; Transmission Assay; Western Blot
bioz_score: 93
MDSD
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