| Catalog No. | Size | Price (USD) |
| 14737-MM04-100 | 100 µL | $251.64 |
General Information
| Product name | Anti-Adenosine Deaminase Antibody, Mouse Monoclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Adenosine Deaminase |
| Immunogen | Recombinant Human Adenosine Deaminase Protein (Catalog#14737-H08B) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Adenosine Deaminase (rh Adenosine Deaminase; Catalog#14737-H08B; P00813; Met1-Leu363). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #04 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-ADA1 Antibody
Image / Experimental Information
| Image 1 Description | ADA was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 µL anti-ADA mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ADA mouse monoclonal antibody,at 1:100 dilution Secondary antibody: Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa Observed band size :41 kDa |
| Image 1 Alt Text | Human Adenosine Deaminase Immunoprecipitation(IP) 43955 |
| Image 1 URL | Source image |
| Image 2 Description | ADA was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 µL anti-ADA mouse monoclonal antibody and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ADA mouse monoclonal antibody,at 1:100 dilution Secondary antibody: Rabbit Anti-Mouse IgG F(ab)2/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 41 kDa Observed band size :41 kDa |
| Image 2 Alt Text | Human Adenosine Deaminase Western blot (WB) 43960 |
| Image 2 URL | Source image |
Background Information
| Full Name | adenosine deaminase |
| Description | Adenosine Desaminase (ADA) deficiency, is a purine metabolic disorder that cause severe combined immunodeficiency (SCID) due to the accumulation of toxic metabolites that primarily affects development, differentiation and function of T and B lymphocytes. Adenosine deaminase is a polymorphic enzyme that has an important role in immune functions and in the regulation of intracellular and extracellular concentrations of adenosine and adenosine receptor activity. ADA activity might be considered as a useful diagnostic tool among the other markers in these diseases. Genetic variability of ADA activity may have, therefore, an important role in resistance to malaria. Adenosine Deaminase (ADA) deficiency is an autosomal recessive variant of severe combined immunodeficiency (SCID) caused by systemic accumulation of ADA substrates. |
