| Catalog No. | Size | Price (USD) |
| 11976-MM34-1 | 1 mL | $1,377.43 |
| 11976-MM34-20 | 20 µL | $88.87 |
| 11976-MM34-50 | 50 µL | $147.96 |
| 11976-MM34-100 | 100 µL | $251.64 |
| 11976-MM34-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Beta-2 microglobulin Antibody, Mouse Monoclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), ELISA(Det) (FAQ Protocol), IHC-P (FAQ Protocol), FCM (FAQ Protocol), ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Beta-2 microglobulin |
| Immunogen | Recombinant Human Beta-2 microglobulin/B2M Protein (Catalog#11976-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Beta-2 microglobulin/B2M (rh Beta-2 microglobulin/B2M; Catalog#11976-H08H; NP_004039.1; Met1-Met119). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #34 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-AMYLD6 Antibody; Anti-CDABP0092 Antibody; Anti-HDCMA22P Antibody; Anti-IMD43 Antibody; Anti-MHC1D4 Antibody
Image / Experimental Information
| Image 1 Description | Anti-B2M mouse monoclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: U937 Whole Cell Lysate Lane C: Raji Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:14 kDa Observed band size:14 kDa |
| Image 1 Alt Text | Human Beta-2 microglobulin Western blot (WB) 20067 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-B2M mouse monoclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: U937 Whole Cell Lysate Lane C: Raji Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:14 kDa Observed band size:14 kDa |
| Image 2 Alt Text | Human Beta-2 microglobulin Immunohistochemistry(IHC) 20068 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-B2M mouse monoclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: U937 Whole Cell Lysate Lane C: Raji Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:14 kDa Observed band size:14 kDa |
| Image 3 Alt Text | Human Beta-2 microglobulin Immunohistochemistry(IHC) 20069 |
| Image 3 URL | Source image |
| Image 4 Description | Anti-B2M mouse monoclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: U937 Whole Cell Lysate Lane C: Raji Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:14 kDa Observed band size:14 kDa |
| Image 4 Alt Text | Human Beta-2 microglobulin Immunofluorescence(IF) 20070 |
| Image 4 URL | Source image |
| Image 5 Description | Anti-B2M mouse monoclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lane B: U937 Whole Cell Lysate Lane C: Raji Whole Cell Lysate Lane D: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:14 kDa Observed band size:14 kDa |
| Image 5 Alt Text | Human Beta-2 microglobulin Flow Cytometry (FC) 21285 |
| Image 5 URL | Source image |
Background Information
| Full Name | beta-2 microglobulin |
| Description | B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the α3 chain. Unlike α3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M (β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (β2 microglobulin) can indicate non-progression of HIV. |
| References |
|
-MM34-Purified-Lymphocytes.png){kind=link}