| Catalog No. | Size | Price (USD) |
| 100166-MCR10-20 | 20 µL | $59.24 |
| 100166-MCR10-100 | 100 µL | $177.73 |
General Information
| Product name | Beta-Actin Loading Control Antibody, Chimeric Rabbit MAb |
| Validated applications | WB (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human beta-Actin |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB |
| Preparation | It is a chimeric monoclonal antibody combining the constant domains of the rabbit IgG molecule with mouse variable regions. The variable region was obtained from a mouse immunized with A synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB. The antibody was produced using recombinant antibody technology. |
| Source | Monoclonal Rabbit IgG Clone #10 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-BKRNS Antibody; Anti-BNS Antibody; Anti-BRWS1 Antibody; Anti-CSMH Antibody; Anti-DDS1 Antibody; Anti-PS1TP5BP1 Antibody; Anti-THC8 Antibody
Image / Experimental Information
| Image 1 Description | Anti-beta-Actin rabbit monoclonal antibody at 1:20000 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lane E: Mouse brain tissue lysate Lane F: Mouse skeletal muscle tissue lysate Lane G: Mouse kidney tissue lysate Lane H: Rat brain tissue lysate Lane I: Rat lung tissue lysate Lane J: Rat kidney tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:42 kDa |
| Image 1 Alt Text | Human beta-Actin Western blot (WB) 42457 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-beta-Actin rabbit monoclonal antibody at 1:20000 dilution Lane A: Jurkat Whole Cell Lysate Lane B: HeLa Whole Cell Lysate Lane C: HepG2 Whole Cell Lysate Lane D: A549 Whole Cell Lysate Lane E: Mouse brain tissue lysate Lane F: Mouse skeletal muscle tissue lysate Lane G: Mouse kidney tissue lysate Lane H: Rat brain tissue lysate Lane I: Rat lung tissue lysate Lane J: Rat kidney tissue lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:42 kDa Observed band size:42 kDa |
| Image 2 Alt Text | Human beta-Actin Immunoprecipitation(IP) 42458 |
| Image 2 URL | Source image |
Background Information
| Full Name | actin, beta |
| Description | ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood. |
title: LncRNA PELATON regulates Th2 cell differentiation by miR-10b-5p/GATA3 axis in allergic rhinitis
authors: Zhen Liu; Yumei Li; ...; Xicheng Song
journal: The World Allergy Organization Journal
date: 2025-09-01
doi: 10.1016/j.waojou.2025.101108
cited_by_count: 0
techniques: Incubation; Protein Extraction; Western Blot
bioz_score: 93
title: Downregulation of exosomal miR-let-7e-5p induces macrophage M2 polarization by targeting Rictor/AKT1 signal pathway in brucellosis patients
authors: Shuling Li; Xiaoyu Zhu; ...; Zhiwei Li
journal: European Journal of Medical Research
date: 2025-07-09
doi: 10.1186/s40001-025-02867-y
cited_by_count: 0
techniques: Activity Assay; BIA-KA; Binding Assay; Cell Culture; Control; Electrophoresis; Expressing; Imaging; Incubation; Luciferase; Lysis; Membrane; Quantitative RT-PCR; Reporter Assay; SDS Page; Transfection; Western Blot
bioz_score: 93
title: Qian Yang Yu Yin Granule-containing serum inhibits angiotensin II-induced proliferation, reactive oxygen species production, and inflammation in human mesangial cells via an NADPH oxidase 4-dependent pathway
authors: Kang Ding; Yan Wang; ...; Zhuyuan Fang
journal: BMC Complementary and Alternative Medicine
date: 2015-03-25
pmid: 25886843
doi: 10.1186/s12906-015-0619-2
pmcid: 4387585
cited_by_count: 14
techniques: Incubation; Software; Western Blot
bioz_score: 93
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