| Catalog No. | Size | Price (USD) |
| 100166-MM10-20 | 20 µL | $88.87 |
| 100166-MM10-50 | 50 µL | $118.49 |
| 100166-MM10-100 | 100 µL | $177.73 |
General Information
| Product name | beta-Actin Loading Control Antibody, Mouse MAb |
| Validated applications | WB (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human, Mouse, Rat |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human beta Actin / ACTB |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the N-terminus of the Human beta Actin / ACTB. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG2a Clone #10 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-BKRNS Antibody; Anti-BNS Antibody; Anti-BRWS1 Antibody; Anti-CSMH Antibody; Anti-DDS1 Antibody; Anti-PS1TP5BP1 Antibody; Anti-THC8 Antibody
Image / Experimental Information
| Image 1 Description | Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: RAW264.7 Whole Cell Lysate Lane D: Mouse slpeen tissue lysate Lane E: Mouse lung tissue lysate Lane F: Mouse brain tissue lysate Lane G: C6 whole cell lysate Lane H: PC-12 whole cell lysate Lane I: Rat liver tissue lysate Lane J: Rat lung tissue lysate Lane K: Rat brain tissue lysate Lysates/proteins at 30 μg per lane. Secondary HRP Conjugated Goat anti-Mouse IgG (H+L) ,at 1:10000 dilution . Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:44 kDa |
| Image 1 Alt Text | Human Beta-Actin Western blot (WB) 18676 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: RAW264.7 Whole Cell Lysate Lane D: Mouse slpeen tissue lysate Lane E: Mouse lung tissue lysate Lane F: Mouse brain tissue lysate Lane G: C6 whole cell lysate Lane H: PC-12 whole cell lysate Lane I: Rat liver tissue lysate Lane J: Rat lung tissue lysate Lane K: Rat brain tissue lysate Lysates/proteins at 30 μg per lane. Secondary HRP Conjugated Goat anti-Mouse IgG (H+L) ,at 1:10000 dilution . Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:44 kDa |
| Image 2 Alt Text | Human Beta-Actin Western blot (WB) 18677 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: RAW264.7 Whole Cell Lysate Lane D: Mouse slpeen tissue lysate Lane E: Mouse lung tissue lysate Lane F: Mouse brain tissue lysate Lane G: C6 whole cell lysate Lane H: PC-12 whole cell lysate Lane I: Rat liver tissue lysate Lane J: Rat lung tissue lysate Lane K: Rat brain tissue lysate Lysates/proteins at 30 μg per lane. Secondary HRP Conjugated Goat anti-Mouse IgG (H+L) ,at 1:10000 dilution . Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:44 kDa |
| Image 3 Alt Text | Human Beta-Actin Immunoprecipitation(IP) 18678 |
| Image 3 URL | Source image |
| Image 4 Description | Anti-beta-Actin mouse monoclonal antibody at 1:2500 dilution Lane A: HepG2 Whole Cell Lysate Lane B: NIH3T3 Whole Cell Lysate Lane C: RAW264.7 Whole Cell Lysate Lane D: Mouse slpeen tissue lysate Lane E: Mouse lung tissue lysate Lane F: Mouse brain tissue lysate Lane G: C6 whole cell lysate Lane H: PC-12 whole cell lysate Lane I: Rat liver tissue lysate Lane J: Rat lung tissue lysate Lane K: Rat brain tissue lysate Lysates/proteins at 30 μg per lane. Secondary HRP Conjugated Goat anti-Mouse IgG (H+L) ,at 1:10000 dilution . Developed using the ECL technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:44 kDa |
| Image 4 Alt Text | Human beta-Actin Western blot (WB) 19962 |
| Image 4 URL | Source image |
Background Information
| Full Name | actin, beta |
| Description | ACTB (Actin Beta) is a Protein Coding gene. Diseases associated with ACTB include Dystonia, Juvenile-Onset and Baraitser-Winter Syndrome 1. Among its related pathways are Salivary secretion and Development Slit-Robo signaling. Gene Ontology (GO) annotations related to this gene include identical protein binding and RNA polymerase II proximal promoter sequence-specific DNA binding. An important paralog of this gene is ACTG1. TUBB, HPRT and ACTB were the most stably expressed genes for all analysis groups across meningioma and non-pathological meningeal tissue combined. ACTB, a significant upregulated gene in abdominal aortic aneurysm samples, could be regulated by CLIC4, which was significantly enriched in cell motions. Dystonia-deafness syndrome is a well-known clinical entity, with sensorineural deafness typically manifesting earlier than dystonia. ACTB p.Arg183Trp heterozygosity has been reported in six patients to cause combined infant-onset deafness and dystonia manifesting in adolescence or young adulthood. |
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