| Catalog No. | Size | Price (USD) |
| 100109-MM05T-20 | 20 µL | $88.87 |
| 100109-MM05T-50 | 50 µL | $118.49 |
| 100109-MM05T-100 | 100 µL | $177.73 |
General Information
| Product name | Beta-Tubulin Loading Control Antibody, Mouse MAb |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol), ICC/IF (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human, Mouse, Rat, Rabbit |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the human beta-tubulin |
| Preparation | This product is a recombinant monoclonal antibody expressed from HEK293 cells. |
| Source | Monoclonal Mouse IgG2b Clone #05 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Immunochemical staining of human Tubulin in human breast carcinoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections). |
| Image 1 Alt Text | Human Beta-Tubulin Immunohistochemistry(IHC) 18429 |
| Image 1 URL | Source image |
| Image 2 Description | Immunochemical staining of human Tubulin in human breast carcinoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections). |
| Image 2 Alt Text | Human Beta-Tubulin Immunohistochemistry(IHC) 18430 |
| Image 2 URL | Source image |
| Image 3 Description | Immunochemical staining of human Tubulin in human breast carcinoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections). |
| Image 3 Alt Text | Human Beta-Tubulin Immunohistochemistry(IHC) 18431 |
| Image 3 URL | Source image |
| Image 4 Description | Immunochemical staining of human Tubulin in human breast carcinoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections). |
| Image 4 Alt Text | Human Beta-Tubulin Immunohistochemistry(IHC) 3002 |
| Image 4 URL | Source image |
| Image 5 Description | Immunochemical staining of human Tubulin in human breast carcinoma with mouse monoclonal antibody (1:20000, formalin-fixed paraffin embedded sections). |
| Image 5 Alt Text | Human Beta-Tubulin Immunoprecipitation(IP) 37649 |
| Image 5 URL | Source image |
Background Information
| Description | Beta-Tubulin is a subunit of tubulin. Tubulin is one of several members of a small family of globular proteins. It is the major constituent of microtubules. There are two most common members of the tubulin family: alpha-tubulin and beta-tubulin, and together their dimers form microtubules. The dimers of alpha- and beta-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. After the dimer is incorporated into the microtubule, the molecule of GTP bound to the beta -tubulin subunit eventually hydrolyzes into GDP through inter-dimer contacts along the microtubule protofilament. Beta-tubulin faces the plus end of the microtubule while alpha-tubulin faces the minus end. Dimers bound to GTP tend to assemble into microtubules, while dimers bound to GDP tend to fall apart. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample you are examining. This ensures constant expression levels. Thus “housekeeping genes” are frequently chosen for use as loading controls. It is also important that the protein chosen as a loading control has a different molecular weight than the protein of interest so that the bands are distinct and expression levels quantifiable. Popular loading control detection antibodies include anti-β-Actin monoclonal or polyclonal antibodies, anti-COX-4, anti-GAPDH, anti-Tubulin and anti-VDAC/Porin antibodies. |
| References |
|
title: An injectable hydrogel for localized sustained release of artesunate in postoperative fibrosis prevention after glaucoma filtration surgery
authors: Guangshuang Tan; Lusi Zhang; ...; Bing Jiang
journal: Drug Delivery
date: 2026-08-03
doi: 10.1080/10717544.2026.2686511
cited_by_count: 0
techniques: Bicinchoninic Acid Protein Assay; Expressing; Molecular Weight; Nucleic Acid Electrophoresis; Software; Western Blot
bioz_score: 94
title: SigmaR1 is an auxiliary translocon factor with lipid-binding activity that regulates protein and lipid droplet homeostasis.
authors: Xuewen Hu; Tiantian Zhou; ...; Wei-Ke Ji
journal: Nature Communications
date: 2026-02-06
pmid: 41476177
cited_by_count: 8
techniques: Immunofluorescence; Western Blot
bioz_score: 94
title: MOSPD3 mediates the ER recruitment of ATG2A to promote autophagy
authors: Du Yuanjiao; Zhou Tiantian; ...; Ji Wei-Ke
journal: bioRxiv
date: 2026-01-04
doi: 10.64898/2026.01.04.697522
cited_by_count: 0
techniques: Concentration Assay; Incubation; Live Cell Imaging; Western Blot
bioz_score: 94
title: Exosomal miR-320b regulates cardiomyocyte FOXM1 expression and may serve as an early-stage compensatory mechanism in obstructive sleep apnea
authors: Wan-yu Wang; Zhi-jia Chen; ...; Chengming Fan
journal: PLoS ONE
date: 2025-09-26
pmid: 41004495
doi: 10.1371/journal.pone.0332862
cited_by_count: 0
techniques: Activity Assay; Binding Assay; Blocking Assay; Construct; Control; Cotransfection; Expressing; Incubation; Luciferase; Mutagenesis; Plasmid Preparation; Quantitative RT-PCR; Reporter Assay; SDS Page; Transfection; Western Blot
bioz_score: 94
title: Chlorogenic acid targets SIRT6 to relieve UVB - induced UV damage.
authors: Simin Wu; Qixing Zhou; ...; Dongying Zhang
journal: Archives of Dermatological Research
date: 2025-05-13
pmid: 40105999
doi: 10.1007/s00403-025-04134-w
cited_by_count: 1
techniques: Activity Assay; Histone Deacetylase Assay; Modification
bioz_score: 94
title: Chitosan-Artesunate nanoparticles: A dual anti-fibrotic and anti-inflammatory strategy for preventing bleb fibrosis post-glaucoma filtration surgery.
authors: Jingyuan Liu; Shutong Wang; ...; Bing Jiang
journal: Drug Delivery and Translational Research
date: 2025-02-28
pmid: 40019651
doi: 10.1007/s13346-025-01819-7
cited_by_count: 2
techniques: Incubation; Membrane; Software; Western Blot
bioz_score: 94
title: A Suite of Foundation Models Captures the Contextual Interplay Between Codons
authors: Naghipourfar Mohsen; Chen Siyu; ...; Goodarzi Hani
journal: bioRxiv
date: 2024-10-13
cited_by_count: 0
techniques: BIA-KA; Blocking Assay; Centrifugation; Control; Expressing; Fluorescence; Functional Assay; Incubation; Membrane; Protease Inhibitor; Protein Concentration; Sequencing; Western Blot
bioz_score: 94
title: Discovery of flavonoid-containing compound Lupalbigenin as anti-NSCLC cancer agents via suppression of EGFR and ERK1/2 pathway.
authors: Xiangdan Cuan; Xingying Yang; ...; Yanping Huang
journal: Bioorganic chemistry
date: 2024-09-07
pmid: 39288634
doi: 10.1016/j.bioorg.2024.107808
cited_by_count: 2
techniques: Proliferation Assay
bioz_score: 94
title: Emodin Inhibits the Indoxyl Sulfate-Induced trans-Differentiation of Vascular Smooth Muscle Cells through Upregulating Thrombospondin-1.
authors: Weidong Zhang; Jing Chen; ...; Shi Jin
journal: Journal of vascular research
date: 2023-10-30
pmid: 37669629
cited_by_count: 2
techniques: Incubation; Membrane
bioz_score: 94
title: Artesunate protects against ocular fibrosis by suppressing fibroblast activation and inducing mitochondria‐dependent ferroptosis
authors: Jingyuan Liu; Zheng Pan; ...; Bing Jiang
journal: The FASEB Journal
date: 2023-05-09
doi: 10.1096/fj.202201867r
cited_by_count: 25
techniques: Bicinchoninic Acid Protein Assay; Concentration Assay; Expressing; Incubation; Marker; Polyacrylamide Gel Electrophoresis; Radio IP Assay; Software; Western Blot
bioz_score: 94
title: Oligomeric CHMP7 mediates three-way ER junctions and ER-mitochondria interactions.
authors: Qingzhu Chu; Jingru Wang; ...; Lin Deng
journal: Cell death and differentiation
date: 2023-01-31
pmid: 35962186
doi: 10.1038/s41418-022-01048-2
cited_by_count: 9
bioz_score: 94
title: Involvement of CXCL17 and GPR35 in Gastric Cancer Initiation and Progression.
authors: Yizhi Li; Aoran Liu; ...; Qian Xu
journal: International Journal of Molecular Sciences
date: 2023-01-10
pmid: 36614059
doi: 10.3390/ijms24010615
cited_by_count: 22
techniques: Bicinchoninic Acid Protein Assay; Blocking Assay; Centrifugation; Incubation; Isolation; Lysis; Transfection; Western Blot
bioz_score: 94
title: Suppression of NK Cell Activation by JAK3 Inhibition: Implication in the Treatment of Autoimmune Diseases
authors: Wai Chung Wu; Carol Shiu; ...; Chin Wai Hui
journal: Journal of immunology research
date: 2023-01-01
pmid: 38106519
doi: 10.1155/2023/8924603
pmcid: 10723930
cited_by_count: 4
techniques: Control; Flow Cytometry
bioz_score: 94
title: A Possible Role of VPS13B in the Formation of Golgi-Lipid Droplet Contacts Associating with the ER.
authors: Yuanjiao Du; Xuewen Hu; ...; Juan Xiong
journal: Contact (Thousand Oaks (Ventura County, Calif.))
date: 2022-10-05
pmid: 38090145
cited_by_count: 7
techniques: Western Blot
bioz_score: 94
title: Interleukins 4 and 21 Protect Anti-IgM Induced Cell Death in Ramos B Cells: Implication for Autoimmune Diseases.
authors: Chin Wai Hui; Wai Chung Wu; Shui On Leung
journal: Frontiers in Immunology
date: 2022-08-02
pmid: 35911775
doi: 10.3389/fimmu.2022.919854
cited_by_count: 5
techniques: Bicinchoninic Acid Protein Assay; Concentration Assay; Lysis; Western Blot
bioz_score: 94
title: A novel mechanism for A-to-I RNA-edited AZIN1 in promoting tumor angiogenesis in colorectal cancer
authors: Yan Wei; Haowan Zhang; ...; Xiaoyan Xu
journal: Cell death & disease
date: 2022-04-02
pmid: 35365616
doi: 10.1038/s41419-022-04734-8
pmcid: 8975946
cited_by_count: 28
techniques: Binding Assay; FLAG-tag; SDS Page; Western Blot
bioz_score: 94
title: The Involvement of CXCL17-GPR35 in Gastric Cancer Initiation and Development
authors: Yi-zhi Li; Ao-ran Liu; ...; Qian Xu
journal: Europe PMC
date: 2021-11-02
doi: 10.21203/rs.3.rs-1029803
cited_by_count: 0
techniques: Bicinchoninic Acid Protein Assay; Blocking Assay; Centrifugation; Expressing; Incubation; Isolation; Lysis; Membrane; Software; Transfection; Western Blot
bioz_score: 94
title: PDZD8-mediated lipid transfer at contacts between the ER and late endosomes/lysosomes is required for neurite outgrowth.
authors: Yuan Gao; Juan Xiong; ...; Wei-Ke Ji
journal: Journal of cell science
date: 2021-06-28
pmid: 33912962
doi: 10.1242/jcs.255026
cited_by_count: 37
techniques: Concentration Assay; Imaging; Immunofluorescence; Incubation; Western Blot
bioz_score: 94
title: An ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D−TSG101 interactions
authors: Jingru Wang; Na Fang; ...; Wei-Ke Ji
journal: Nature Communications
date: 2021-02-23
pmid: 33623047
doi: 10.1038/s41467-021-21525-5
pmcid: 7902631
cited_by_count: 78
techniques: Immunofluorescence; Western Blot
bioz_score: 94
title: Trans-Golgi network-lipid droplet contacts maintain the TGN integrity and function via lipid transfer activities of VPS13B
authors: Du Yuanjiao; Xiong Juan; Ji Wei-Ke
journal: bioRxiv
date: 2020-12-16
doi: 10.1101/2020.12.16.423147
cited_by_count: 0
techniques: Electron Microscopy; Immunofluorescence; Western Blot
bioz_score: 94
title: BAP1 suppresses prostate cancer progression by deubiquitinating and stabilizing PTEN
authors: Rong Deng; Yanmin Guo; ...; Jianxiu Yu
journal: Molecular Oncology
date: 2020-11-20
pmid: 33155366
doi: 10.1002/1878-0261.12844
pmcid: 7782096
cited_by_count: 39
bioz_score: 94
title: β 2 ‐adrenergic receptor signaling drives prostate cancer progression by targeting the Sonic hedgehog‐Gli1 signaling activation
authors: Mi Zhang; Qianhui Wang; ...; Chen Xu
journal: The Prostate
date: 2020-09-07
pmid: 32894788
pmcid: 7540401
cited_by_count: 7
techniques: Immunohistochemistry; Incubation; Western Blot
bioz_score: 94
title: Caffeine Targets SIRT3 to Enhance SOD2 Activity in Mitochondria
authors: Huanhuan Xu; Chunxia Gan; ...; Jun Sheng
journal: Frontiers in Cell and Developmental Biology
date: 2020-09-01
pmid: 33015038
doi: 10.3389/fcell.2020.00822
pmcid: 7493682
cited_by_count: 35
techniques: Activity Assay; Screening Assay; Staining
bioz_score: 94
title: ATG3, a Target of miR-431-5p, Promotes Proliferation and Invasion of Colon Cancer via Promoting Autophagy
authors: Wei Huang; Chong Zeng; ...; Jie Liu
journal: Cancer Management and Research
date: 2019-12-09
pmid: 31849517
doi: 10.2147/CMAR.S226828
pmcid: 6911302
cited_by_count: 29
techniques: Incubation; Western Blot
bioz_score: 94
title: <p>ATG3, a Target of miR-431-5p, Promotes Proliferation and Invasion of Colon Cancer via Promoting Autophagy</p>
authors: Wei Huang; Chong Zeng; ...; Jie Liu
journal: Cancer Management and Research
date: 2019-12-01
doi: 10.2147/cmar.s226828
cited_by_count: 28
techniques: Construct; Incubation; Luciferase; Reporter Assay
bioz_score: 94
title: Dihydroorotate dehydrogenase (DHODH) inhibitors affect ATP depletion, endogenous ROS and mediate S-phase arrest in breast cancer cells.
authors: A K Mohamad Fairus; B Choudhary; ...; O Shatrah
journal: Biochimie
date: 2017-03-21
pmid: 28196676
doi: 10.1016/j.biochi.2017.02.003
cited_by_count: 92
bioz_score: 94
