| Catalog No. | Size | Price (USD) |
| 12285-T52-50 | 50 µL | $147.96 |
| 12285-T52-100 | 100 µL | $251.64 |
| 12285-T52-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Carbonic Anhydrase VB Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Carbonic Anhydrase VB |
| Immunogen | Recombinant Human Carbonic Anhydrase VB / CA5B Protein (Catalog#12285-H08E) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human Carbonic Anhydrase VB / CA5B (rh Carbonic Anhydrase VB / CA5B; Catalog#12285-H08E; Q9Y2D0; Cys34-Pro317). Carbonic Anhydrase VB / CA5B specific IgG was purified by Human Carbonic Anhydrase VB / CA5B affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CA-VB Antibody; Anti-CAVB Antibody
Image / Experimental Information
| Image 1 Description | CA5B was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate 2 µL anti-CA5B rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CA5B rabbit polyclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size: 35 kDa |
| Image 1 Alt Text | Human Carbonic Anhydrase VB Immunoprecipitation(IP) 3476 |
| Image 1 URL | Source image |
| Image 2 Description | CA5B was immunoprecipitated using: Lane A:0.5 mg MCF-7 Whole Cell Lysate 2 µL anti-CA5B rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-CA5B rabbit polyclonal antibody,at 1:200 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 35 kDa Observed band size: 35 kDa |
| Image 2 Alt Text | Human Carbonic Anhydrase VB Western blot (WB) 13483 |
| Image 2 URL | Source image |
Background Information
| Full Name | carbonic anhydrase VB, mitochondrial |
| Description | Carbonic anhydrase 5B, also known as carbonate dehydratase VB, carbonic anhydrase VB, CA-VB and CA5B, is amember of the alpha-carbonic anhydrase family. The strongest expression of CA5B / CA-VB is in heart, pancreas, kidney, placenta, lung, and skeletal muscle. It is not expressed in liver. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CAs show extensive diversity in tissue distribution and in their subcellular localization. CA5B / CA-VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA5A / CA-VA. CA5B / CA-VB has a wider tissue distribution than CA5A / CA-VA, which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt). CA5B / CA-VB is inhibited by coumarins, sulfonamide derivatives such as acetazolamide (AZA), saccharin and Foscarnet (phosphonoformate trisodium salt). |
| References |
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