| Catalog No. | Size | Price (USD) |
| 10617-RP02-50 | 50 µL | $147.96 |
| 10617-RP02-100 | 100 µL | $251.64 |
| 10617-RP02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-carbonic anhydrase XII/CA12 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human carbonic anhydrase XII/CA12 |
| Immunogen | Recombinant Human CA12 protein (Catalog#10617-H08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human CA12 extracellular domain (rh CA12; Catalog#10617-H08H; NP_001209.1; Met 1-Gln 291). CA12 specific IgG was purified by human CA12 affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CA-XII Antibody; Anti-CAXII Antibody; Anti-HsT18816 Antibody; Anti-T18816 Antibody
Image / Experimental Information
| Image 1 Description | Immunochemical staining of human CA12 in human prostatic carcinomawith rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. |
| Image 1 Alt Text | Human Carbonic Anhydrase XII Immunohistochemistry(IHC) 4634 |
| Image 1 URL | Source image |
| Image 2 Description | Immunochemical staining of human CA12 in human prostatic carcinomawith rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. |
| Image 2 Alt Text | Human Carbonic Anhydrase XII Immunohistochemistry(IHC) 4635 |
| Image 2 URL | Source image |
| Image 3 Description | Immunochemical staining of human CA12 in human prostatic carcinomawith rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. |
| Image 3 Alt Text | Human Carbonic Anhydrase XII Immunohistochemistry(IHC) 4636 |
| Image 3 URL | Source image |
| Image 4 Description | Immunochemical staining of human CA12 in human prostatic carcinomawith rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. |
| Image 4 Alt Text | Human Carbonic Anhydrase XII Western blot (WB) 13255 |
| Image 4 URL | Source image |
Background Information
| Full Name | carbonic anhydrase XII |
| Description | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity. |
| References |
|
title: Converting Double-Stranded DNA-Encoded Libraries (DELs) to Single-Stranded Libraries for More Versatile Selections
authors: Yuhan Gui; Clara Shania Wong; ...; Xiaoyu Li
journal: ACS Omega
date: 2022-03-24
pmid: 35415338
doi: 10.1021/acsomega.2c01152
pmcid: 8992267
cited_by_count: 4
techniques: Concentration Assay; Electrophoresis; Mass Spectrometry; Purification; Sequencing
bioz_score: 90
title: Selection of DNA-encoded chemical libraries against endogenous membrane proteins on live cells.
authors: Yiran Huang; Ling Meng; ...; Xiaoyu Li
journal: Nature Chemistry
date: 2021-03-25
pmid: 33349694
doi: 10.1038/s41557-020-00605-x
cited_by_count: 108
techniques: Immunohistochemistry; Recombinant; Western Blot
bioz_score: 90
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