Recombinant Anti-Carboxypeptidase M Antibody, Rabbit Monoclonal
Validated applications
WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol)
Species reactivity
Reacts with: Human
Specificity
Human Carboxypeptidase M
Immunogen
Recombinant Human Carboxypeptidase M / CPM Protein (Catalog#11228-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human Carboxypeptidase M / CPM (rh Carboxypeptidase M / CPM; Catalog#11228-H08H; NP_938079.1; Met 1-His 422).
Source
Monoclonal Rabbit IgG Clone #105
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Image / Experimental Information
Image 1 Description
Anti-CPM rabbit monoclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:51 kDa Observed band size:55 kDa (We are unsure as to the identity of these extra bands.)
Image 1 Alt Text
Human Carboxypeptidase M/CPM Western blot (WB) 14779
Anti-CPM rabbit monoclonal antibody at 1:500 dilution Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:51 kDa Observed band size:55 kDa (We are unsure as to the identity of these extra bands.)
Image 2 Alt Text
Human Carboxypeptidase M/CPM Immunoprecipitation(IP) 14817
Carboxypeptidase M, also known as CPM, is a membrane-bound arginine/lysine carboxypeptidase which is a member of the carboxypeptidases family. These enzymes remove C-terminal amino acids from peptides and proteins and exert roles in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing. Among the carboxypeptidases CPM is of particular importance because of its constitutive expression in an active form at the surface of specialized cells and tissues in the human body. CPM in the brain appears to be membrane-bound via a phosphatidylinositol glycan anchor. CPM is widely distributed in a variety of tissues and cells. The amino acid sequence of CPM indicated that the C-terminal hydrophobic region might be a signal for membrane attachment via a glycosylphosphatidylinositol (GPI) anchor. CPM is involved in peptide metabolism on both the cell surface and in extracellular fluids. CPM functions not only as a protease but also as a binding partner in cell-surface protein-protein interactions.
