| Catalog No. | Size | Price (USD) |
| 14434-R104-50 | 50 µL | $177.58 |
| 14434-R104-100 | 100 µL | $340.51 |
General Information
| Product name | Recombinant Anti-FHIT Antibody, Rabbit Monoclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human FHIT |
| Immunogen | Recombinant Human Fragile histidine triad / FHIT Protein (Catalog#14434-H08E) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Human Fragile histidine triad / FHIT (rh Fragile histidine triad / FHIT; Catalog#14434-H08E; P49789; Met1-Gln147). |
| Source | Monoclonal Rabbit IgG Clone #104 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-AP3Aase Antibody; Anti-FRA3B Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of Human FHIT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FHIT monoclonal antibody (1:50) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). |
| Image 1 Alt Text | Human Fragile histidine triad / FHIT Immunohistochemistry(IHC) 2922 |
| Image 1 URL | Source image |
| Image 2 Description | Immunofluorescence staining of Human FHIT in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FHIT monoclonal antibody (1:50) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). |
| Image 2 Alt Text | Human Fragile histidine triad / FHIT Western blot (WB) 6988 |
| Image 2 URL | Source image |
Background Information
| Full Name | fragile histidine triad |
| Description | Fragile histidine triad, also known as FHIT, may play a key role in differentiating humans from apes. Fragile histidine triad gene belongs to the histidine triad gene family. It has been shown that fragile histidine triad synergizes with VHL, another tumor suppressor, in protecting against chemically - induced lung cancer. Fragile histidine triad gene works as a tumor suppressor as it has been demonstrated in animal studies. The exact molecular function of FHIT is still partially unclear. It also acts as a tumor suppressor of HER2/neu driven breast cancer. |
| Research Areas |
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| References |
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title: LRP1 involvement in FHIT-regulated HER2 signaling in non-small cell lung cancer.
authors: Théophile Ponchel; Emma Loeffler; ...; Béatrice Nawrocki-Raby
journal: European journal of cell biology
date: 2024-12-26
pmid: 39752787
doi: 10.1016/j.ejcb.2024.151475
cited_by_count: 0
techniques: Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Proximity Ligation Assay; Western Blot
bioz_score: 92
