Human Mouse (Species predicted to react based on 100% sequence homology)
Immunogen
A synthetic peptide corresponding to the N-terminus of the human H3F3A
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the human H3F3A, and purified by antigen affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Image / Experimental Information
Image 1 Description
H3F3A was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg K562 Whole Cell Lysate 2 µL anti-H3F3A rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-H3F3A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 15 kDa Observed band size: 15 kDa
H3F3A was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate Lane B:0.5 mg K562 Whole Cell Lysate 2 µL anti-H3F3A rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-H3F3A rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 15 kDa Observed band size: 15 kDa
H3-3A (H3.3 Histone A, also known as H3F3A, H3F3, H3-3B, and H3.3A) is a Protein Coding gene. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. H3F3A constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. H3F3A is widely expressed in bone marrow, appendix, and other tissues. Diseases associated with H3-3A include Glioma Susceptibility 1 and Chondroblastoma. A mutation in the H3 histone family member 3A gene (H3F3A) was recently identified as a genetic signature for giant cell tumor of bone (GCTB).
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