| Catalog No. | Size | Price (USD) |
| 12283-T26-20 | 20 µL | $88.87 |
| 12283-T26-50 | 50 µL | $147.96 |
| 12283-T26-100 | 100 µL | $251.64 |
| 12283-T26-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-CD47 Antibody, Rabbit Polyclonal |
| Validated applications | ELISA (FAQ Protocol), IHC-P (FAQ Protocol), ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human, Cynomolgus |
| Specificity | Human, Cynomolgus |
| Immunogen | Recombinant Human CD47 Protein (Catalog#12283-H08H) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human CD47 (rh CD47; Catalog#12283-H08H; NP_942088.1; Met1-Pro139). CD47 specific IgG was purified by Human CD47 affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-IAP Antibody; Anti-MER6 Antibody; Anti-OA3 Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of CD47 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CD47 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cell membrane. |
| Image 1 Alt Text | Human CD47 Immunofluorescence(IF) 20579 |
| Image 1 URL | Source image |
| Image 2 Description | Immunofluorescence staining of CD47 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CD47 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cell membrane. |
| Image 2 Alt Text | Human CD47 Immunohistochemistry(IHC) 20641 |
| Image 2 URL | Source image |
| Image 3 Description | Immunofluorescence staining of CD47 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CD47 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cell membrane. |
| Image 3 Alt Text | Human CD47 Immunohistochemistry(IHC) 20642 |
| Image 3 URL | Source image |
| Image 4 Description | Immunofluorescence staining of CD47 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CD47 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cell membrane. |
| Image 4 Alt Text | Human CD47 Immunohistochemistry(IHC) 21236 |
| Image 4 URL | Source image |
| Image 5 Description | Immunofluorescence staining of CD47 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human CD47 polyclonal antibody (dilution ratio 1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to cell membrane. |
| Image 5 Alt Text | Human CD47 Immunohistochemistry(IHC) 21254 |
| Image 5 URL | Source image |
Background Information
| Full Name | CD47 molecule |
| Description | CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus. |
| Research Areas |
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| Tags |
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| References |
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title: Multi-omics Research on the Heterogeneity and Immune Landscape of Lung Adenocarcinoma with Ground-glass opacity
authors: Huiwen Miao; Xiao Teng; ...; Heyun Xu
journal: Europe PMC
date: 2022-10-21
doi: 10.21203/rs.3.rs-2155357
cited_by_count: 0
techniques: Immunohistochemical staining; Immunohistochemistry; Standard Deviation
bioz_score: 90
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