| Catalog No. | Size | Price (USD) |
| 10004-MM03-20 | 20 µL | $88.87 |
| 10004-MM03-50 | 50 µL | $147.96 |
| 10004-MM03-100 | 100 µL | $251.64 |
| 10004-MM03-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Her2/ERBB2 Antibody, Mouse Monoclonal |
| Validated applications | FCM (FAQ Protocol), ICC/IF (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Her2/ERBB2 Has cross-reactivity in ELISA with Human HER2 / ErbB2 Protein (ECD,1-652) (Cat# 10004-HCCH) Human HER2 / ErbB2 Protein (ECD,1-652) (His Tag) (Cat# 10004-H08H) No cross-reactivity in ELISA with Human HER2 / ErbB2 Protein (ECD, domain I) (His Tag) (Cat# 10004-H08H1) Human HER2 / ErbB2 Protein (ECD, domain IV) (His Tag) (Cat# 10004-H08H4) Human HER2 / ErbB2 Protein (Cytoplasmic Domain, 676-1255) (His & GST Tag) (Cat# 10004-H20B1) |
| Immunogen | Recombinant Human HER2 / ErbB2 protein (Catalog#10004-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HER2 / ErbB2 (rh HER2 / ErbB2; Catalog#10004-H08H; NP_004439.2; Met1-Thr652). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #03 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS with 10% Trehalose, pH7.0 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-c-ERB-2 Antibody; Anti-c-ERB2 Antibody; Anti-CD340 Antibody; Anti-HER-2 Antibody; Anti-HER-2/neu Antibody; Anti-HER2 Antibody; Anti-MLN 19 Antibody; Anti-MLN-19 Antibody; Anti-MLN19 Antibody; Anti-NEU Antibody; Anti-NGL Antibody; Anti-p185(erbB2) Antibody; Anti-TKR1 Antibody; Anti-VSCN2 Antibody
Image / Experimental Information
| Image 1 Description | Immunofluorescence staining of Human ERBB2 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with mouse anti-Human ERBB2 monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cell membrane. |
| Image 1 Alt Text | Human HER2/ERBB2 Immunohistochemistry(IHC) 2932 |
| Image 1 URL | Source image |
| Image 2 Description | Immunofluorescence staining of Human ERBB2 in MCF7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with mouse anti-Human ERBB2 monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cell membrane. |
| Image 2 Alt Text | Human HER2/ERBB2 Flow Cytometry (FC) 5435 |
| Image 2 URL | Source image |
Background Information
| Full Name | erb-b2 receptor tyrosine kinase 2 |
| Description | Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on. |
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| References |
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title: Label-Free Multiplex SERS Microfluidic Immunoassay of Breast Cancer Biomarkers in Serum Using Directionally Conjugated mAb-Gold Nanourchins on a PCB-Thin Gold Film Platform
authors: Mohammad E. Khosroshahi; Gayathri Senthilchelvan; ...; Victor Oyebolu
journal: Plasmonics
date: 2025-12-23
doi: 10.1007/s11468-025-03332-6
cited_by_count: 0
techniques: Bioprocessing
bioz_score: 93
title: Microfluidic Nanosensor for Label-Free Multiplexed Detection of Breast Cancer Biomarkers via Surface-Enhanced Reflective FTIR Spectroscopy Using Thin Gold Films and Antibody-Oriented Gold Nanourchin: Feasibility Study
authors: Mohammad E. Khosroshahi; Gayathri Senthilchelvan; ...; Hugo Aguas
journal: Micromachines
date: 2025-11-11
pmid: 41302786
doi: 10.3390/mi16111268
cited_by_count: 0
techniques: Bioprocessing
bioz_score: 93
title: Dual-Oriented Targeted Nanostructured SERS Label-Free Immunosensor for Detection, Quantification, and Analysis of Breast Cancer Biomarker Concentrations in Blood Serum
authors: Mohammad E. Khosroshahi; Christine Gaoiran; ...; Pranav Panday
journal: Biosensors
date: 2025-07-11
pmid: 40710097
doi: 10.3390/bios15070447
cited_by_count: 1
techniques: Bioprocessing; Conjugation Assay
bioz_score: 93
title: Single-cell HER2 quantification via instant signal amplification in microdroplets.
authors: Xiaoxian Liu; Yifan Zhu; ...; Guanghui Wang
journal: Analytica Chimica Acta
date: 2023-03-20
pmid: 36925278
doi: 10.1016/j.aca.2023.340976
cited_by_count: 4
bioz_score: 93
title: Antibody binding reports spatial heterogeneities in cell membrane organization
authors: Arnold Daniel P.; Xu Yaxin; Takatori Sho C.
journal: bioRxiv
date: 2022-11-22
doi: 10.1101/2022.11.21.517201
cited_by_count: 0
techniques: Labeling; Recombinant
bioz_score: 93
title: para-Sulfonatocalix[4]arene stabilized gold nanoparticles multilayers interfaced to electrodes through host-guest interaction for sensitive ErbB2 detection.
authors: Xingxin Wang; Dongshu Du; ...; Hongxia Chen
journal: Biosensors & bioelectronics
date: 2018-06-12
pmid: 28802750
doi: 10.1016/j.bios.2017.08.011
cited_by_count: 18
bioz_score: 93
title: Surface plasmon resonance immunosensor for ErbB2 breast cancer biomarker determination in human serum and raw cancer cell lysates
authors: Unai Eletxigerra; Josu Martinez-Perdiguero; ...; Santos Merino
journal: Analytica Chimica Acta
date: 2016-01-01
doi: 10.1016/j.aca.2015.12.020
cited_by_count: 93
techniques: Bioprocessing
bioz_score: 93
