| Catalog No. | Size | Price (USD) |
| 16008-MM02-H-20 | 20 µg | $118.49 |
| 16008-MM02-H-100 | 100 µg | $296.22 |
General Information
| Product name | Anti-Human IgG1 F(ab)2 Antibody (HRP), Mouse MAb |
| Validated applications | ELISA (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Recognize the Human IgG1 full-length antibody and F(ab)2 in ELISA, don't recognize the Human IgG2, Human IgG4, and Human IgG Fc region. |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography and conjugated with horseradish-peroxidase (HRP). |
| Source | Monoclonal Mouse IgG1 Clone #02 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | HRP |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Protected from prolonged exposure to light. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. |
| References |
|
title: Humanized anti-VEGF antibody Fab fragment
authors: Liangzhi Xie; Chunyun Sun; ...; Desheng Kong
date: 2025-11-25
cited_by_count: 0
techniques: Binding Assay; Concentration Assay; Incubation; Negative Control; Recombinant; Software
bioz_score: 93
title: Human anti-IFN-α antibodies
authors: Syeda F. Y. Haque; Adrian Hayday; ...; Martin Woodward
date: 2024-04-02
cited_by_count: 0
techniques: Activity Assay; Binding Assay; Blocking Assay; Enzyme-linked Immunosorbent Assay; Incubation; Recombinant
bioz_score: 93
title: An engineered bispecific nanobody in tetrameric secretory IgA format confers broad neutralization against SARS-CoV-1&2 and most variants.
authors: Weihong Zeng; Xiaoying Jia; ...; Tengchuan Jin
journal: International Journal of Biological Macromolecules
date: 2023-11-24
pmid: 37690653
doi: 10.1016/j.ijbiomac.2023.126817
cited_by_count: 7
techniques: Activity Assay; Binding Assay; Concentration Assay; Enzyme-linked Immunosorbent Assay; Incubation; Inhibition; Molecular Weight; Mutagenesis; Neutralization; Purification; SDS Page; Size-exclusion Chromatography; Software
bioz_score: 91
title: Monoclonal enolase-1 blocking antibody ameliorates pulmonary inflammation and fibrosis.
authors: Wei-Ching Huang; Chi-Fen Chuang; ...; Ta-Tung Yuan
journal: Respiratory research
date: 2023-11-14
pmid: 37964270
doi: 10.1186/s12931-023-02583-3
cited_by_count: 2
techniques: Cell Culture; Recombinant
bioz_score: 92
title: Raman spectroscopy characterization of antibody phases in serum
authors: Audrey E Baker; Amber R Mantz; Mark L Chiu
journal: mAbs
date: 2014-11-02
pmid: 25484036
doi: 10.4161/19420862.2014.975100
pmcid: 4622053
cited_by_count: 13
techniques: Produced; Protein Concentration; Stable Transfection
bioz_score: 91
title: The receptors CD96 and CD226 oppose each other in the regulation of natural killer cell functions.
authors: Christopher J Chan; Ludovic Martinet; ...; Mark J Smyth
journal: Nature immunology
date: 2014-07-09
pmid: 24658051
doi: 10.1038/ni.2850
cited_by_count: 376
techniques: Blocking Assay; Flow Cytometry; Injection; Isolation; Labeling; Produced; Recombinant; Staining
bioz_score: 93
