| Catalog No. | Size | Price (USD) |
| CTA15-1 | 1 mg | $231.05 |
| CTA15-200 | 200 µg | $96.27 |
General Information
| Product name | Recombinant Anti-IgG1-Fc Antibody (Biotin), Rabbit Monoclonal |
| Validated applications | ELISA (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human IgG |
| Immunogen | Recombinant Human IgG |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Human IgG1, and then biotinylated. |
| Source | Monoclonal Rabbit IgG |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Biotin |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Description | As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5 kDa ) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcγR ) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. |
| References |
|
title: Overcoming steric inhibition of antibody-dependent phagocytosis with tall adhesions
authors: Joffe Aaron M.; Chorlay Aymeric; ...; Fletcher Daniel A.
journal: bioRxiv
date: 2026-01-10
doi: 10.64898/2026.01.09.698749
cited_by_count: 0
techniques: Binding Assay; Comparison; Flow Cytometry; Fluorescence; Incubation; Labeling; Live Cell Imaging; Negative Control; Positive Control; Protein Concentration
bioz_score: 94
title: Colorimetric and Raman dual-mode lateral flow immunoassay detection of SARS-CoV-2 N protein antibody based on Ag nanoparticles with ultrathin Au shell assembled onto Fe 3 O 4 nanoparticles.
authors: Jingwen Li; Penghui Liang; ...; Jingbin Zeng
journal: Analytical and Bioanalytical Chemistry
date: 2023-02-15
pmid: 36414739
cited_by_count: 6
bioz_score: 94
title: Ag Nanoparticles with Ultrathin Au Shell-Based Lateral Flow Immunoassay for Colorimetric and SERS Dual-Mode Detection of SARS-CoV-2 IgG
authors: Penghui Liang; Qi Guo; ...; Jingbin Zeng
journal: Analytical Chemistry
date: 2022-06-03
pmid: 35657150
doi: 10.1021/acs.analchem.2c01286
pmcid: 9211040
cited_by_count: 37
bioz_score: 94
title: Microfluidic system evaluation for the semi-automatic detection of MOG-IgG in serum samples
authors: Numfon Khemthongcharoen; Panapat Uawithya; ...; Chamras Promptmas
journal: Sensing and Bio-Sensing Research
date: 2021-12-01
doi: 10.1016/j.sbsr.2021.100458
cited_by_count: 1
techniques: Staining
bioz_score: 94
title: HER2 antigenic polypeptide compositions, and methods for their use in treatment and prevention of carcinomas
authors: Wei-Zen Wei; Heather Gibson; ...; Joyce Reyes
date: 2019-10-08
cited_by_count: 0
techniques: Cell Culture; Enzyme-linked Immunosorbent Assay; Enzyme-linked Immunospot; In Vitro; Incubation; Isolation; Plasmid Preparation; Recombinant; Transfection
bioz_score: 94
title: Antibodies to the cytoplasmic domain of PD-L1 most clearly delineate cell membranes in immunohistochemical staining
authors: Kathleen M. Mahoney; Heather Sun; ...; Gordon J. Freeman
journal: Cancer Immunology Research
date: 2016-12-01
pmid: 26546452
doi: 10.1158/2326-6066.CIR-15-0116
pmcid: 4743889
cited_by_count: 106
bioz_score: 94
title: Induction of HER2 Immunity in Outbred Domestic Cats by DNA Electrovaccination
authors: Heather M. Gibson; Jesse J. Veenstra; ...; Wei-Zen Wei
journal: Cancer Immunology Research
date: 2015-02-23
doi: 10.1158/2326-6066.cir-14-0175
cited_by_count: 13
techniques: Cloning; Enzyme-linked Immunosorbent Assay
bioz_score: 94
