| Catalog No. | Size | Price (USD) |
| 11905-MM01-50 | 50 µL | $147.96 |
| 11905-MM01-100 | 100 µL | $251.64 |
| 11905-MM01-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-IRE1 Antibody, Mouse Monoclonal |
| Validated applications | ELISA (FAQ Protocol), IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human IRE1 No cross-reactivity in ELISA with Insect cell lysate |
| Immunogen | Recombinant Human ERN1 / IRE1 protein (Catalog#11905-H20B) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ERN1 / IRE1 (rh ERN1 / IRE1; Catalog#11905-H20B; O75460-1; Pro 465-Leu 977). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG2b Clone #10B2B2 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-hIRE1p Antibody; Anti-IRE1 Antibody; Anti-IRE1a Antibody; Anti-IRE1P Antibody
Image / Experimental Information
| Image 1 Description | Immunochemical staining of human ERN1 in human tonsil with mouse monoclonal antibody (1:300, formalin-fixed paraffin embedded sections). |
| Image 1 Alt Text | Human IRE1/ERN1 Immunohistochemistry(IHC) 4216 |
| Image 1 URL | Source image |
| Image 2 Description | Immunochemical staining of human ERN1 in human tonsil with mouse monoclonal antibody (1:300, formalin-fixed paraffin embedded sections). |
| Image 2 Alt Text | Human IRE1/ERN1 Immunohistochemistry(IHC) 4217 |
| Image 2 URL | Source image |
| Image 3 Description | Immunochemical staining of human ERN1 in human tonsil with mouse monoclonal antibody (1:300, formalin-fixed paraffin embedded sections). |
| Image 3 Alt Text | Human IRE1/ERN1 Immunohistochemistry(IHC) 4218 |
| Image 3 URL | Source image |
Background Information
| Full Name | endoplasmic reticulum to nucleus signaling 1 |
| Description | Endoplasmic reticulum stress and hypoxia are necessary components of malignant tumors growth and suppression of ERN1 (from endoplasmic reticulum to nuclei-1) signalling pathway, which is linked to the apoptosis and cell death processes, significantly decreases proliferative processes. An enhanced expression of TP53 gene in ERN1 knockdown glioma cells correlates with the decreased level of ubiquitin ligase MDM2 and increased expression level of USP7 which deubiquitinates TP53 and MDM2 and induces TP53-dependent cell growth repression and apoptosis. Thus, the expression of genes encoding TP53 and related to TP53 factors depends upon the endoplasmic reticulum stress signaling as well as on hypoxia, and correlates with suppression of glioma growth under ERN1 knockdown. The dependence of insulin-like growth binding proteins as well as IGF2BP3 and HTRA1 gene expressions in U87 glioma cells on ERN1 signaling enzyme function and hypoxia, indicating its participation in the regulation of metabolic and proliferative processes via IGF/INS receptors, because endoplasmic reticulum stress is an important component of tumor growth and metabolic diseases. |
| References |
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