| Catalog No. | Size | Price (USD) |
| 104245-T32-50 | 50 µL | $147.96 |
| 104245-T32-100 | 100 µL | $251.64 |
| 104245-T32-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-LRRC8D Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human LRRC8D |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human LRRC8D |
| Preparation | Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human LRRC8D, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | PBS, pH7.0 with 0.03% Proclin300 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-FLJ10470 Antibody; Anti-HsLRRC8D Antibody; Anti-LRRC5 Antibody; Anti-UNQ213/PRO239 Antibody
Image / Experimental Information
| Image 1 Description | Anti-LRRC8D rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:98 kDa |
| Image 1 Alt Text | Human LRRC8D Western blot (WB) 19768 |
| Image 1 URL | Source image |
Background Information
| Description | Members of the leucine-rich repeat-containing 8 (LRRC8) protein family, composed of the five LRRC8A-E isoforms, are pore-forming components of the volume-regulated anion channel (VRAC). LRRC8A and at least one of the other LRRC8 isoforms assemble into heteromers to generate VRAC transport activities. Among the five LRRC8 isoforms, the LRRC8D isoform is the largest one owing to a long first extracellular loop between the transmembrane (TM) 1 and 2 helices. When incorporated into LRRC8 hetero-hexamers, the LRRC8D isoform facilitates the permeation of uncharged substrates such as taurine and GABA, negatively charged glutamate, and even positively charged lysine. Moreover, LRRC8D-containing hetero-hexamers facilitate the uptake of the antibiotic blasticidin S and the Pt-based anticancer drug cisplatin, resulting in cancer drug resistance in patients with down-regulated LRRC8D isoforms. |
| References |
|
title: LRRC8D Suppresses Prostate Cancer Growth and Enhances Platinum Sensitivity via Modulation of CAV-1/STAT3 Signaling
authors: Rong Xu; Xue Shui; ...; Changhong Shi
journal: Membranes
date: 2026-06-08
doi: 10.3390/membranes16060198
cited_by_count: 0
techniques: Imaging; Western Blot
bioz_score: 94
title: Regulation of volume-regulated anion channels alters sensitivity to platinum chemotherapy
authors: Lily Elizabeth R. Feldman; Saswat Mohapatra; ...; Dan Theodorescu
journal: Science Advances
date: 2024-12-13
pmcid: 11641020
cited_by_count: 0
techniques: CRISPR; Control; Liquid Chromatography with Mass Spectroscopy; Software; Western Blot
bioz_score: 92
title: NPEPPS regulates intracellular import and sensitivity to cisplatin by interaction with volume regulated anion channels
authors: Jones Robert T.; Goodspeed Andrew; ...; Costello James C.
journal: bioRxiv
date: 2021-06-19
doi: 10.1101/2021.03.04.433676
cited_by_count: 0
techniques: Activity Assay; Amplification; CRISPR; Expressing; Immunoprecipitation; Incubation; Knock-Out; Labeling; Over Expression; Western Blot; shRNA
bioz_score: 92
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