| Catalog No. | Size | Price (USD) |
| 12302-MM04-F-25 | 25 Tests | $103.68 |
| 12302-MM04-F-100 | 100 Tests | $325.84 |
General Information
| Product name | Anti-MICA Antibody (FITC), Mouse Monoclonal |
| Validated applications | FCM (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human MICA |
| Immunogen | Recombinant Human MICA protein (Catalog#12302-H08H) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human MICA (rh MICA; Catalog#12302-H08H; AAH16929.1; Met 1-Gln 308) and conjugated with FITC under optimum conditions, the unreacted FITC was removed. |
| Source | Monoclonal Mouse IgG1 Clone #04 |
| Purification | Protein A |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Concentration | 10 μl/Test, 0.1 mg/ml |
| Conjugate | FITC |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! |
Synonyms: Anti-MIC-A Antibody; Anti-PERB11.1 Antibody
Image / Experimental Information
| Image 1 Description | Profile of anti-MICA reactivity on HeLa cells analyzed by flow cytometry. |
| Image 1 Alt Text | Human MICA Flow Cytometry (FC) 2568 |
| Image 1 URL | Source image |
Background Information
| Full Name | MHC class I polypeptide-related sequence A |
| Description | MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to the MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules do not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gamma-delta T cells, CD8+ alpha-beta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by the ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gamma-delta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems. |
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