Phospho-Myosin Light Chain 2 (Thr18, Ser19) Antibody, Rabbit MAb
Validated applications
WB (FAQ Protocol)
Species reactivity
Reacts with: Human Predicted to React with: This antbody also react with Human Phospho-Myosin Light Chain 2 (Thr18), which correspond to Phospho-Myosin Light Chain 2 (Thr19) of P24844-1 in UniProt in ELISA. Human Species predicted to react based on 100% sequence homology: Mouse, Cynomolgus, Bovine, Pig
Specificity
Human Phospho-Myosin Light Chain 2 (Thr18, Ser19), which correspond to Phospho-Myosin Light Chain 2 (Thr19, Ser20) of P24844-1 in UniProt. This antbody also react with Human Phospho-Myosin Light Chain 2 (Thr18), which correspond to Phospho-Myosin Light Chain 2 (Thr19) of P24844-1 in UniProt in ELISA. Human Species predicted to react based on 100% sequence homology: Mouse, Cynomolgus, Bovine, Pig
Immunogen
A synthetic peptide corresponding to the residues around (Thr18, Ser19) of Human Phospho-Myosin Light Chain 2
Preparation
This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the residues around (Thr18, Ser19) of Human Phospho-Myosin Light Chain 2.
Source
Monoclonal Rabbit IgG Clone #0010
Purification
Protein A
Formulation
10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western blot analysis of extracts from serum-starved Hela, untreated (line A) or treated with CalyculinA (100nM, 30min)(line B), using Phospho-Myosin Light Chain 2 (Thr18, Ser19) rabbit monoclonal Antibody (Cat# 111556-R0010) at 1:10000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal (Cat# 100166-MCR10) at 1:50000 dilution (lower).
Western blot analysis of extracts from serum-starved Hela, untreated (line A) or treated with CalyculinA (100nM, 30min)(line B), using Phospho-Myosin Light Chain 2 (Thr18, Ser19) rabbit monoclonal Antibody (Cat# 111556-R0010) at 1:10000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal (Cat# 100166-MCR10) at 1:50000 dilution (lower).