A synthetic phosphopeptide corresponding to residues around Thr35 of the Human Phospho-MOB1
Preparation
This antibody was obtained from a rabbit immunized with a synthetic phosphopeptide corresponding to residues around Thr35 of the Human Phospho-MOB1.
Source
Monoclonal Rabbit IgG Clone #0004
Purification
Protein A
Formulation
10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL BSA, 50% glycerol, pH 7.5
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
Western blot analysis of extracts from serum-starved HepG2, untreated(line A) or treated with Calyculin A (100ng/mL, 60min; +)(line B), using Phospho-MOB1 (Thr35) rabbit monoclonal Antibody (Cat: 112042-R0004) at 1:50000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal (Cat: 100166-MCR10) at 1:200000 dilution (lower).
Western blot analysis of extracts from serum-starved HepG2, untreated(line A) or treated with Calyculin A (100ng/mL, 60min; +)(line B), using Phospho-MOB1 (Thr35) rabbit monoclonal Antibody (Cat: 112042-R0004) at 1:50000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal (Cat: 100166-MCR10) at 1:200000 dilution (lower).
Western blot analysis of extracts from serum-starved HepG2, untreated(line A) or treated with Calyculin A (100ng/mL, 60min; +)(line B), using Phospho-MOB1 (Thr35) rabbit monoclonal Antibody (Cat: 112042-R0004) at 1:50000 dilution (upper) or Anti-Actin Antibody, Chimeric Rabbit Monoclonal (Cat: 100166-MCR10) at 1:200000 dilution (lower).