| Catalog No. | Size | Price (USD) |
| 100508-MM03-50 | 50 µL | $147.96 |
| 100508-MM03-100 | 100 µL | $251.64 |
| 100508-MM03-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-S100B Antibody, Mouse Monoclonal |
| Validated applications | IHC-P (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human Mouse, Rat (Species predicted to react based on 100% sequence homology) |
| Immunogen | A synthetic peptide corresponding to the N-terminus of the Human S100B |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with a synthetic peptide corresponding to the N-terminus of the Human S100B . The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG1 Clone #03 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-NEF Antibody; Anti-S100 Antibody; Anti-S100-B Antibody; Anti-S100beta Antibody
Image / Experimental Information
| Image 1 Description | Immunochemical staining of human S100B in human malignant melanoma with mouse monoclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections. |
| Image 1 Alt Text | Human S100B Immunohistochemistry(IHC) 19100 |
| Image 1 URL | Source image |
| Image 2 Description | Immunochemical staining of human S100B in human malignant melanoma with mouse monoclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections. |
| Image 2 Alt Text | Human S100B Immunohistochemistry(IHC) 19101 |
| Image 2 URL | Source image |
| Image 3 Description | Immunochemical staining of human S100B in human malignant melanoma with mouse monoclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections. |
| Image 3 Alt Text | Human S100B Immunohistochemistry(IHC) 19102 |
| Image 3 URL | Source image |
Background Information
| Full Name | S100 calcium binding protein B |
| Description | S100B is a member of the S100 family of proteins containing two EF-hand-type calcium-binding motifs. S100B exerts both intracellular and extracellular functions. Intracellular S100B acts as a stimulator of cell proliferation and migration and an inhibitor of apoptosis and differentiation, which might have important implications during brain, cartilage and skeletal muscle development and repair, activation of astrocytes in the course of brain damage and neurodegenerative processes, and of cardiomyocyte remodeling after infarction, as well as in melanomagenesis and gliomagenesis. As an extracellular factor, S100B engages RAGE (receptor for advanced glycation end products) in a variety of cell types with different outcomes (i.e. beneficial or detrimental, pro-proliferative or pro-differentiative) depending on the concentration attained by the protein, the cell type and the microenvironment. This calcium binding astrocyte-specific cytokine, presents a marker of astrocytic activation and reflects CNS injury. The excellent sensitivity of S100B has enabled it to confirm the existence of subtle brain injury in patients with mild head trauma, strokes, and after successful resuscitation from cardiopulmonary arrest. Recent findings provide evidence, that S100B may decrease neuronal injury and/or contribute to repair following traumatic brain injury (TBI). Hence, S100B, far from being a negative determinant of outcome, as suggested previously in the human TBI and ischemia literature, is of potential therapeutic value that could improve outcome in patients who sustain various forms of acute brain damage. |
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