| Catalog No. | Size | Price (USD) |
| 10339-RP02-50 | 50 µL | $147.96 |
| 10339-RP02-100 | 100 µL | $251.64 |
| 10339-RP02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-SMAC Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Human |
| Specificity | Human SMAC |
| Immunogen | Recombinant Human Diablo protein (Catalog#10339-H08E) |
| Preparation | Produced in rabbits immunized with purified, recombinant Human Diablo (rh Diablo; Catalog#10339-H08E; NP_063940.1; Ala 56-Asp 239). Diablo specific IgG was purified by human Diablo affinity chromatography . |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-DFNA64 Antibody; Anti-DIABLO-S Antibody; Anti-FLJ10537 Antibody; Anti-FLJ25049 Antibody; Anti-SMAC Antibody
Image / Experimental Information
| Image 1 Description | Diablo was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate Lane C:0.5 mg MCF-7 Whole Cell Lysate Lane D:0.5 mg SH-SY5Y Whole Cell Lysate 2 µL anti-Diablo rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-Diablo rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 19 kDa Observed band size: 19 kDa |
| Image 1 Alt Text | Human SMAC/Diablo Immunoprecipitation(IP) 7975 |
| Image 1 URL | Source image |
| Image 2 Description | Diablo was immunoprecipitated using: Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate Lane C:0.5 mg MCF-7 Whole Cell Lysate Lane D:0.5 mg SH-SY5Y Whole Cell Lysate 2 µL anti-Diablo rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose. Primary antibody: Anti-Diablo rabbit polyclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 19 kDa Observed band size: 19 kDa |
| Image 2 Alt Text | Human SMAC/Diablo Western blot (WB) 13193 |
| Image 2 URL | Source image |
Background Information
| Full Name | diablo, IAP-binding mitochondrial protein |
| Description | Apoptosis is an essential processes required for normal development and homeostasis of all metazoan organisms. Second Mitochondria-Derived Activator of Caspases (Smac) or Direct IAP Binding Protein with low isoelectric point, pI (Diablo) is a proapoptogenic mitochondrial protein that is released to the cytosol in response to diverse apoptotic stimuli, including commonly used chemotherapeutic drugs. The current knowlege about structure and function of Smac/Diablo during programmed cell death, both in mitochondrial and receptor pathways are presented. It has been shown that Diablo mainly interacts with IAPs in the cytochrome c/Apaf-1/caspase-9 pathway, and promotes apoptosis. Diablo is released from the mitochondria into the cytosol occurring downstream of cytochrome c release in response to apoptotic stimuli such as irradiation, DNA damage or cytotoxic drugs. In the cytosol, Smac/Diablo interacts and antagonizes inhibitors of apoptosis proteins (IAPs), thus allowing the activation of caspases and apoptosis. This activity has prompted the synthesis of peptidomimetics that could potentially be used in cancer therapy. The role of Smac/DIABLO in colorectal carcinogenesis is ill defined. Data continues to accumulate to suggest that decreased levels of Smac/DIABLO may be important in chemoradiation-resistance to apoptosis in advanced colon cancer. |
| Related Pathways |
|
| References |
|
title: Vanillin ameliorates changes in HIF-1α expression and neuronal apoptosis in a rat model of spinal cord injury
authors: Hailong Chen; Jiuqin Zheng; Junjie Ma
journal: Restorative Neurology and Neuroscience
date: 2019-03-06
doi: 10.3233/rnn-180879
cited_by_count: 3
techniques: BIA-KA; Blocking Assay; Immunohistochemical staining; Immunohistochemistry; In Situ; Incubation; Isolation; Staining; TUNEL Assay; Western Blot
bioz_score: 90
-HB04NO2406-IP.png){kind=link}
