| Catalog No. | Size | Price (USD) |
| 11973-MM05T-P-25 | 25 Tests | $177.73 |
| 11973-MM05T-P-100 | 100 Tests | $473.95 |
General Information
| Product name | Anti-M13 Antibody (PE), Mouse Monoclonal |
| Validated applications | FCM (FAQ Protocol) |
| Species reactivity | Reacts with: other |
| Specificity | The antibody binds specifically to phage coat proteins of fd phage or M13 phage |
| Immunogen | M13 Bacteriophage |
| Preparation | This product is a recombinant monoclonal antibody expressed from HEK293 cells. Then conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Source | Monoclonal Mouse IgG1 Clone #05 |
| Purification | Protein A |
| Formulation | PBS solution containing 0.5% BSA and 0.03%ProClin300 |
| Concentration | 10 μl/Test, 0.1 mg/ml |
| Conjugate | PE |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! |
Image / Experimental Information
| Image 1 Description | 4-1BB-transfected CHO-K1 cells were incubated with M13 phage particles for 20 minutes, then detected with 10 μL phage M13-PE Ab (Cat# 11973-MM05T-P). The cell line stained with a 4-1BB specific M13 phage antibody (filied profile). Compared to an irrelevant phage antibody (open profile).The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| Image 1 Alt Text | other M13 Flow Cytometry (FC) 30693 |
| Image 1 URL | Source image |
Background Information
| Description | M13 is a filamentous bacteriophage composed of circular single-stranded DNA (ssDNA) which is 647 nucleotides long encapsulated in approximately 27 copies of the major coat protein P8 and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. Infection with filamentous phages is not lethal, however, the infection causes turbid plaques in E. coli. It is a non-lytic virus. However, a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology. The phage coat is primarily assembled from a 5 amino acid protein called pVIII (or p8), which is encoded by gene VIII (or g8) in the phage genome. For a wild type M13 particle, it takes about approximately 27 copies of p8 to make the coat about 9 nm long. The coat's dimensions are flexible though and the number of p8 copies adjusts to accommodate the size of the single-stranded genome it packages. The general stages to a viral life cycle are infection, replication of the viral genome, assembly of new viral particles, and then release of the progeny particles from the host. Filamentous phage uses a bacterial structure known as the F pilus to infect E. coli, with the M13 p3 tip contacting the TolA protein on the bacterial pilus. The phage genome is then transferred to the cytoplasm of the bacterial cell where resident proteins convert the single-stranded DNA genome to a double-stranded replicative form. |
| References |
|
title: Autoantibody biomarkers and first-line therapy response in RA: findings from the CAP48 cohort.
authors: Sukayna Fadlallah; Judith Fraussen; ...; Veerle Somers
journal: RMD Open
date: 2026-03-13
pmid: 41825926
doi: 10.1136/rmdopen-2025-006407
cited_by_count: 0
techniques: Binding Assay; Enzyme-linked Immunosorbent Assay
bioz_score: 94
title: Development of a Novel Probe-Based Dual-Detection platform System for Flexible Detection of Staphylococcus aureus across Transmission Pathways.
authors: Shengyang Wang; Wenjin Hu; ...; Yaling Liu
journal: Analytical Chemistry
date: 2026-03-09
pmid: 41801772
doi: 10.1021/acs.analchem.5c07055
cited_by_count: 0
bioz_score: 94
title: Dendritic cell-targeted liposomes for cancer immunotherapy via inhibition of aryl hydrocarbon receptor
authors: Chen-Guang Zhang; Chen-Yun Yeh; ...; Yungling Leo Lee
journal: Journal of Nanobiotechnology
date: 2025-10-15
pmid: 41088149
cited_by_count: 2
techniques: Countercurrent Chromatography; Flow Cytometry; Immunofluorescence; Immunopeptidomics; Western Blot
bioz_score: 94
title: Universal Dual-Target CAR-γδT Cells Targeting B7-H3 and IL13Rα2 for Glioblastoma Therapy
authors: Xu Yang; Ye Yunhao; ...; Liu Guangna
journal: bioRxiv
date: 2025-05-16
doi: 10.1101/2025.05.14.653035
cited_by_count: 0
techniques: Binding Assay; Biomarker Discovery; Clone Assay; Enzyme-linked Immunosorbent Assay; Extraction; Flow Cytometry; Incubation; Magnetic Beads; Plasmid Preparation; Sequencing
bioz_score: 94
title: Monoclonal antibody specifically binding to LAG-3 and use thereof
authors: Sang Pil Lee; Ji-Young Shin; ...; Young Woo Park
date: 2024-04-02
cited_by_count: 0
techniques: Binding Assay; Enzyme-linked Immunosorbent Assay; Selection; Spectrophotometry
bioz_score: 94
title: Screening, Expression and Identification of Nanobody Against Monkeypox Virus A35R
journal: International Journal of Nanomedicine
date: 2023-12-01
doi: 10.2147/ijn.s431619
cited_by_count: 0
bioz_score: 94
title: Large-scale phosphomimetic screening identifies phospho-modulated motif-based protein interactions.
authors: Johanna Kliche; Dimitriya Hristoforova Garvanska; ...; Ylva Ivarsson
journal: Molecular Systems Biology
date: 2023-07-12
pmid: 37219487
doi: 10.15252/msb.202211164
cited_by_count: 28
techniques: Enzyme-linked Immunosorbent Assay; Incubation
bioz_score: 94
title: Promoting immunity with novel targeting antigen delivery vehicle based on bispecific nanobody.
authors: Haiwei Cheng; Li Yang; ...; Qisheng Zheng
journal: International Immunopharmacology
date: 2023-06-01
pmid: 37116343
doi: 10.1016/j.intimp.2023.110140
cited_by_count: 4
techniques: Plasmid Preparation; Recombinant
bioz_score: 94
title: A PD-1 peptide antagonist exhibits potent anti-tumor and immune regulatory activity.
authors: Huimin Tao; Lu Cheng; ...; Zheng Qiu
journal: Cancer letters
date: 2021-03-25
pmid: 32805322
doi: 10.1016/j.canlet.2020.08.009
cited_by_count: 16
techniques: Activation Assay; Binding Assay; Cell Culture; Enzyme-linked Immunosorbent Assay; Flow Cytometry; Labeling; Marker; Purification; Stable Transfection; Staining; Transfection
bioz_score: 94
title: Aggregates in blood filter chambers used from the plasma donations of anti-D donors: evaluation for monoclonal antibody discovery using phage display
authors: Eunike C. McGowan; Robert L. Flower; ...; Xuan T. Bui
journal: Blood Transfusion
date: 2021-01-01
pmid: 33085594
doi: 10.2450/2020.0093-20
pmcid: 7850920
cited_by_count: 3
techniques: Clone Assay; Flow Cytometry; Positive Control; Software
bioz_score: 94
