| Catalog No. | Size | Price (USD) |
| 106198-T44-50 | 50 µL | $147.96 |
| 106198-T44-100 | 100 µL | $251.64 |
| 106198-T44-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-Cytokeratin 14/KRT14 Antibody, Rabbit Polyclonal |
| Validated applications | WB (FAQ Protocol), IHC-P (FAQ Protocol), IP (FAQ Protocol) |
| Species reactivity | Reacts with: Mouse |
| Specificity | Mouse Cytokeratin 14/KRT14 |
| Immunogen | A synthetic peptide corresponding to the center region of the Mouse KRT14 |
| Preparation | Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Mouse KRT14, and purified by antigen affinity chromatography. |
| Source | Polyclonal Rabbit IgG |
| Purification | Protein A & Antigen Affinity |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Synonyms: Anti-CK-14 Antibody; Anti-K14 Antibody; Anti-Krt-1.14 Antibody; Anti-Krt1-14 Antibody; Anti-Krt14 Antibody
Image / Experimental Information
| Image 1 Description | Anti-KRT14 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:53 kDa Observed band size:53 kDa |
| Image 1 Alt Text | Mouse Cytokeratin 14 Western blot (WB) 20252 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-KRT14 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:53 kDa Observed band size:53 kDa |
| Image 2 Alt Text | Mouse Cytokeratin 14 Immunohistochemistry(IHC) 20253 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-KRT14 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:53 kDa Observed band size:53 kDa |
| Image 3 Alt Text | Mouse Cytokeratin 14 Immunohistochemistry(IHC) 20254 |
| Image 3 URL | Source image |
| Image 4 Description | Anti-KRT14 rabbit polyclonal antibody at 1:500 dilution Lane A: A431 Whole Cell Lysate Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:53 kDa Observed band size:53 kDa |
| Image 4 Alt Text | Mouse Cytokeratin 14 Immunoprecipitation(IP) 20255 |
| Image 4 URL | Source image |
Background Information
| Full Name | keratin 14, type I |
| Description | Cytokeratin 14, also known as Keratin 14 and K14, is a member of the keratin family. Cytokeratin 14 is a type I keratin. It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Cytokeratin 14 is mainly expressed in the basal layer. It is also strongly expressed in the outer root sheath of anagen follicles. Cytokeratin 14 and keratin 5 may have a role in the maintenance of cell proliferation potential in the basal layer of stratified epithelia, modulating phosphatidylinositol 3-kinase/Akt–mediated cell proliferation and/or Notch1-dependent cell differentiation. Cytokeratin 14 defect prevents it from working effectively with keratin 5 and interfering with the assembly of the keratin intermediate filament network. A disruption in this network makes keratinocytes fragile and prone to rupture. Minor trauma to the skin, such as rubbing or scratching, can cause these cells to break down, resulting in the formation of painful, fluid-filled blisters. Mutations in the K14 gene are also responsible for Naegeli-Franceschetti-Jadassohn syndrome and Dermatopathia Pigmentosa Reticularis. |
| References |
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