| Catalog No. | Size | Price (USD) |
| 50440-R009-P-25 | 25 Tests | $103.68 |
| 50440-R009-P-100 | 100 Tests | $222.17 |
General Information
| Product name | Recombinant Anti-ICAM-1/CD54 Antibody (PE), Rabbit Monoclonal |
| Validated applications | FCM (FAQ Protocol) |
| Species reactivity | Reacts with: Mouse |
| Specificity | Mouse ICAM-1/CD54 |
| Immunogen | Recombinant Mouse ICAM-1 protein (Catalog#50440-M08H) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD54 / ICAM1 (rM CD54 / ICAM1; Catalog#50440-M08H; NP_034623.1; Met 1-Asn 485) and conjugated with PE under optimum conditions, the unreacted PE was removed. |
| Source | Monoclonal Rabbit IgG Clone #009 |
| Purification | Protein A |
| Formulation | PBS solution containing 0.5% BSA and 0.1% sodium azide |
| Concentration | 10 μl/Test, 0.1 mg/ml |
| Conjugate | PE |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. |
Synonyms: Anti-CD54 Antibody; Anti-Icam-1 Antibody; Anti-Icam1 Antibody; Anti-Ly-47 Antibody; Anti-MALA-2 Antibody
Image / Experimental Information
| Image 1 Description | Analysis of ICAM1 (CD54) expression on spleen lymphocytes. Naive BALB/c splenocytes were treated with Mouse BD Fc Block™ purified anti-CD16/CD32 mAb 2.4G2 (Cat. No. 553141) and stained with PE-conjugated anti-Mouse ICAM1 (CD54) (50440-R009-P). The histogram were derived from the gated events based on light scattering characteristics of lymphocytes. |
| Image 1 Alt Text | Mouse ICAM-1/CD54 Flow Cytometry (FC) 4986 |
| Image 1 URL | Source image |
Background Information
| Full Name | intercellular adhesion molecule 1 |
| Description | Intercellular adhesion molecule-1 (ICAM-1, or CD54) is a 90 kDa member of the immunoglobulin (Ig) superfamily and is critical for the firm arrest and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is constitutively present on endothelial cells, but its expression is increased by proinflammatory cytokines. The endothelial expression of ICAM-1 is increased in atherosclerotic and transplant-associated atherosclerotic tissue and animal models of atherosclerosis. Additionally, ICAM-1 has been implicated in the progression of autoimmune diseases. ICAM-1 is a ligand for LFA-1(integrin). When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 interaction and then transmigrate into tissues. Presence with heavy glycosylation and other structural characteristics, ICAM-1 possesses binding sites for some immune-associated ligands and serves as the binding site for entry of the major group of human Rhinovirus (HRV) into various cell types. ICAM-1 also becomes known for its affinity for Plasmodium falciparum-infected erythrocytes (PFIE), providing more of a role in infectious disease. Previous studies have shown that ICAM-1 is involved in inflammatory reactions and that a defect in ICAM-1 gene inhibits allergic contact hypersensitivity. |
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