| Catalog No. | Size | Price (USD) |
| 40438-R016-20 | 20 µg | $236.98 |
| 40438-R016-100 | 100 µg | $592.44 |
General Information
| Product name | Pan Influenza B Nucleoprotein Antibody, Rabbit MAb |
| Validated applications | WB (FAQ Protocol), ELISA (FAQ Protocol) |
| Application notes | IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
| Specificity | Widely recognize Influenza B Nucleoprotein Has cross-reactivity in ELISA and WB with Influenza B (B/Austria/1359417/2021) NP Protein (Cat# 40861-V08B) Influenza B (B/Brisbane/60/2008) NP (Catalog# 40783-V08B) Influenza B (B/Colorado/06/2017) NP (Catalog# 40782-V08B) Influenza B (B/Florida/4/2006) NP (Catalog# 40438-V08B) Influenza B (B/Phuket/3073/2013) NP (Catalog# 40500-V08B) Influenza B (B/Washington/02/2019) NP (Catalog# 40755-V08B) Has cross-reactivity in ELISA with Influenza B (B/Victoria/705/2018) NP (Catalog# 40854-V08B) No cross-reactivity in ELISA and WB with H1N1 (A/Brevig Mission/1/1918) NP (Catalog# 40204-V08B) H1N1 (A/Brisbane/02/2018 NP (Catalog# 40776-V08B) H1N1 (A/California/07/2009) NP (Catalog# 40205-V08B) H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP (Catalog# 40723-V08B) H1N1 (A/Hawaii/70/2019) NP (Catalog# 40724-V08B) H1N1 (A/Michigan/45/2015 NP (Catalog# 40777-V08B) H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M) (Catalog# 11675-V08B) H1N1 (A/Victoria/2570/2019)/(A/Wisconsin/588/2019) NP (Catalog# 40774-V08B) H2N2 (A/Ann Arbor/6/1960) NP (Catalog# 40033-V08B) H3N2 (A/Aichi/2/1968) NP (Catalog# 40207-V08B) H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP (Catalog# 40778-V08B) H3N2 (A/Darwin/9/2021)/(A/Darwin/6/2021) NP Protein (Cat# 40858-V08B) H3N2 (A/Hong Kong/1/1968) NP (Catalog# 40208-V08B) H3N2 (A/Hong Kong/2671/2019) NP (Catalog# 40753-V08B) H3N2 (A/Hong Kong/45/2019) NP (Catalog# 40754-V08B) H3N2 (A/Hong Kong/4801/2014) NP (Catalog# 40781-V08B) H3N2 (A/Kansas/14/2017) NP (Catalog# 40779-V08B) H3N2 (A/Switzerland/9715293/2013) NP (Catalog# 40499-V08B) H5N1 (A/Texas/37/2024) NP Protein (Cat# 41014-V07E) H7N9 (A/Anhui/1-BALF_RG6/2013) NP (Catalog# 40110-V08B) H7N9 (A/Shanghai/2/2013) NP (Catalog# 40111-V08B) No cross-reactivity in ELISA with H1N1 (A/Victoria/2454/2019) NP (Catalog# 40851-V08B) SARS-CoV-2 Nucleocapsid Protein (Catalog# 40588-V08B) HCoV-229E Nucleocapsid Protein (Catalog# 40640-V07E) HCoV-NL63 Nucleocapsid Protein (Catalog# 40641-V07E) HCoV-HKU1 Nucleocapsid Protein (Catalog# 40642-V07E) HCoV-OC43 Nucleocapsid Protein (Catalog# 40643-V07E) |
| Immunogen | Recombinant Influenza B (B/Florida/4/2006) Nucleoprotein / NP Protein (Catalog#40438-V08B) |
| Preparation | This antibody was obtained from a rabbit immunized with purified, recombinant Influenza B (B/Florida/4/2006) Nucleoprotein / NP Protein (Catalog# 40438-V08B; ACF54251.1; Met1-Tyr560). |
| Source | Monoclonal Rabbit IgG Clone #016 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Image / Experimental Information
| Image 1 Description | Anti-NP rabbit monoclonal antibody at 1:2000 dilution. Sample: Recombinant Protein 10 ng Lane 1: H1N1 (A/Brevig Mission/1/1918) NP (Catalog# 40204-V08B) Lane 2: H1N1 (A/Brisbane/02/2018 NP (Catalog# 40776-V08B) Lane 3: H1N1 (A/California/07/2009) NP (Catalog# 40205-V08B) Lane 4: H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP (Catalog# 40723-V08B) Lane 5: H1N1 (A/Hawaii/70/2019) NP (Catalog# 40724-V08B) Lane 6: H1N1 (A/Michigan/45/2015 NP (Catalog# 40777-V08B) Lane 7: H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M) (Catalog# 11675-V08B) Lane 8:H1N1 (A/Victoria/2570/2019A/Wisconsin/588/2019 NP (Catalog# 40774-V08B) Lane 9: H2N2 (A/Ann Arbor/6/1960) NP (Catalog# 40033-V08B) Lane 10: H3N2 (A/Aichi/2/1968) NP (Catalog# 40207-V08B) Lane 11: H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP (Catalog# 40778-V08B) Lane 12: H3N2 (A/Hong Kong/1/1968) NP (Catalog# 40208-V08B) Lane 13: H3N2 (A/Hong Kong/2671/2019) NP (Catalog# 40753-V08B) Lane 14: H3N2 (A/Hong Kong/45/2019) NP (Catalog# 40754-V08B) Lane 15: H3N2 (A/Hong Kong/4801/2014 NP (Catalog# 40781-V08B) Lane 16: H3N2 (A/Kansas/14/2017 NP (Catalog# 40779-V08B) Lane 17: H3N2 (A/Switzerland/9715293/2013) NP (Catalog# 40499-V08B) Lane 18: H7N9 (A/Anhui/1-BALF_RG6/2013) NP (Catalog# 40110-V08B) Lane 19: H7N9 (A/Shanghai/2/2013) NP (Catalog# 40111-V08B) Lane 20: Influenza B (B/Brisbane/60/2008 NP (Catalog# 40783-V08B) Lane 21: Influenza B (B/Colorado/06/2017 NP (Catalog# 40782-V08B) Lane 22: Influenza B (B/Florida/4/2006) NP (Catalog# 40438-V08B) Lane 23: Influenza B (B/Phuket/3073/2013) NP (Catalog# 40500-V08B) Lane 24: Influenza B (B/Washington/02/2019) NP (Catalog# 40755-V08B) Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. |
| Image 1 Alt Text | Influenza B Nucleocapsid/NP Western blot (WB) 32250 |
| Image 1 URL | Source image |
| Image 2 Description | Anti-NP rabbit monoclonal antibody at 1:2000 dilution. Sample: Recombinant Protein 10 ng Lane 1: H1N1 (A/Brevig Mission/1/1918) NP (Catalog# 40204-V08B) Lane 2: H1N1 (A/Brisbane/02/2018 NP (Catalog# 40776-V08B) Lane 3: H1N1 (A/California/07/2009) NP (Catalog# 40205-V08B) Lane 4: H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP (Catalog# 40723-V08B) Lane 5: H1N1 (A/Hawaii/70/2019) NP (Catalog# 40724-V08B) Lane 6: H1N1 (A/Michigan/45/2015 NP (Catalog# 40777-V08B) Lane 7: H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M) (Catalog# 11675-V08B) Lane 8:H1N1 (A/Victoria/2570/2019A/Wisconsin/588/2019 NP (Catalog# 40774-V08B) Lane 9: H2N2 (A/Ann Arbor/6/1960) NP (Catalog# 40033-V08B) Lane 10: H3N2 (A/Aichi/2/1968) NP (Catalog# 40207-V08B) Lane 11: H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP (Catalog# 40778-V08B) Lane 12: H3N2 (A/Hong Kong/1/1968) NP (Catalog# 40208-V08B) Lane 13: H3N2 (A/Hong Kong/2671/2019) NP (Catalog# 40753-V08B) Lane 14: H3N2 (A/Hong Kong/45/2019) NP (Catalog# 40754-V08B) Lane 15: H3N2 (A/Hong Kong/4801/2014 NP (Catalog# 40781-V08B) Lane 16: H3N2 (A/Kansas/14/2017 NP (Catalog# 40779-V08B) Lane 17: H3N2 (A/Switzerland/9715293/2013) NP (Catalog# 40499-V08B) Lane 18: H7N9 (A/Anhui/1-BALF_RG6/2013) NP (Catalog# 40110-V08B) Lane 19: H7N9 (A/Shanghai/2/2013) NP (Catalog# 40111-V08B) Lane 20: Influenza B (B/Brisbane/60/2008 NP (Catalog# 40783-V08B) Lane 21: Influenza B (B/Colorado/06/2017 NP (Catalog# 40782-V08B) Lane 22: Influenza B (B/Florida/4/2006) NP (Catalog# 40438-V08B) Lane 23: Influenza B (B/Phuket/3073/2013) NP (Catalog# 40500-V08B) Lane 24: Influenza B (B/Washington/02/2019) NP (Catalog# 40755-V08B) Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. |
| Image 2 Alt Text | Influenza B Nucleoprotein/NP Western blot (WB) 34376 |
| Image 2 URL | Source image |
| Image 3 Description | Anti-NP rabbit monoclonal antibody at 1:2000 dilution. Sample: Recombinant Protein 10 ng Lane 1: H1N1 (A/Brevig Mission/1/1918) NP (Catalog# 40204-V08B) Lane 2: H1N1 (A/Brisbane/02/2018 NP (Catalog# 40776-V08B) Lane 3: H1N1 (A/California/07/2009) NP (Catalog# 40205-V08B) Lane 4: H1N1 (A/Guangdong-Maonan/SWL1536/2019) NP (Catalog# 40723-V08B) Lane 5: H1N1 (A/Hawaii/70/2019) NP (Catalog# 40724-V08B) Lane 6: H1N1 (A/Michigan/45/2015 NP (Catalog# 40777-V08B) Lane 7: H1N1 (A/Puerto Rico/8/34/Mount Sinai) NP (I116M) (Catalog# 11675-V08B) Lane 8:H1N1 (A/Victoria/2570/2019A/Wisconsin/588/2019 NP (Catalog# 40774-V08B) Lane 9: H2N2 (A/Ann Arbor/6/1960) NP (Catalog# 40033-V08B) Lane 10: H3N2 (A/Aichi/2/1968) NP (Catalog# 40207-V08B) Lane 11: H3N2 (A/Cambodia/e0826360/2020 (H3N2)-like NP (Catalog# 40778-V08B) Lane 12: H3N2 (A/Hong Kong/1/1968) NP (Catalog# 40208-V08B) Lane 13: H3N2 (A/Hong Kong/2671/2019) NP (Catalog# 40753-V08B) Lane 14: H3N2 (A/Hong Kong/45/2019) NP (Catalog# 40754-V08B) Lane 15: H3N2 (A/Hong Kong/4801/2014 NP (Catalog# 40781-V08B) Lane 16: H3N2 (A/Kansas/14/2017 NP (Catalog# 40779-V08B) Lane 17: H3N2 (A/Switzerland/9715293/2013) NP (Catalog# 40499-V08B) Lane 18: H7N9 (A/Anhui/1-BALF_RG6/2013) NP (Catalog# 40110-V08B) Lane 19: H7N9 (A/Shanghai/2/2013) NP (Catalog# 40111-V08B) Lane 20: Influenza B (B/Brisbane/60/2008 NP (Catalog# 40783-V08B) Lane 21: Influenza B (B/Colorado/06/2017 NP (Catalog# 40782-V08B) Lane 22: Influenza B (B/Florida/4/2006) NP (Catalog# 40438-V08B) Lane 23: Influenza B (B/Phuket/3073/2013) NP (Catalog# 40500-V08B) Lane 24: Influenza B (B/Washington/02/2019) NP (Catalog# 40755-V08B) Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. |
| Image 3 Alt Text | Influenza B Nucleoprotein/NP Western blot (WB) 42783 |
| Image 3 URL | Source image |
Background Information
| Description | Influenza viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. Nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins. |
title: A bovine adenoviral-vector-based universal influenza vaccine confers protection against influenza A and B viruses in mice and ferrets
authors: Wen-Chien Wang; Ekramy E. Sayedahmed; ...; Suresh K. Mittal
journal: Molecular Therapy. Nucleic Acids
date: 2025-09-09
pmid: 40635672
doi: 10.1016/j.omtn.2025.102594
cited_by_count: 1
techniques: Imaging; Incubation; Marker; Membrane; Western Blot
bioz_score: 93
