Anti-ART4/CD297 Antibody, Rabbit Polyclonal sinobiological 80383-T24

$143.00
In stock
SKU
80383-T24
Catalog No.SizePrice (USD)
80383-T24-5050 µL$147.96
80383-T24-100100 µL$251.64
80383-T24-200200 µL$355.32

General Information

Product nameAnti-ART4/CD297 Antibody, Rabbit Polyclonal
Validated applicationsELISA (FAQ Protocol), IHC-P (FAQ Protocol)
Species reactivityReacts with: Rat
SpecificityRat ART4/CD297
ImmunogenRecombinant Rat ART4 Protein (Catalog#80383-R08H)
PreparationProduced in rabbits immunized with purified, recombinant Rat ART4 (rR ART4; Catalog#80383-R08H; NP_001166980.1; Met1-Lys269). ART4 specific IgG was purified by Rat ART4 affinity chromatography.
SourcePolyclonal Rabbit IgG
PurificationProtein A & Antigen Affinity
Formulation0.2 μm filtered solution in PBS
ConjugateUnconjugated
FormLiquid
ShippingThis antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
StorageThis antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Synonyms: Anti-ATR4 Antibody

Image / Experimental Information

Image 1 DescriptionImmunochemical staining of rat ART4 in rat kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Image 1 Alt TextRat CD297 Immunohistochemistry(IHC) 3149
Image 1 URLSource image

Background Information

Full NameADP-ribosyltransferase 4 (Dombrock blood group)
DescriptionADP-ribosyltransferase 4 (Dombrock blood group), also known as Mono-ADP-ribosyltransferase 4(ART4), Dombrock blood group carrier molecule and CD297, is a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. ADP-ribosyltransferase catalyzes the ADP-ribosylation of arginine residues in proteins. Mono-ADP-ribosylation is a posttranslational modification of proteins that is interfered with by a variety of bacterial toxins including cholera, pertussis, and heat-labile enterotoxins of E. coli. ART4 could be detected on HEL cells and erythrocytes by FACS analysis while it was absent from activated monocytes, despite the presence of ART4 mRNA in these cells. ART is also known as the carrier of the Dombrock blood group alloantigens (Do) which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane.
References
  1. Parusel I, et al. (2005) A panel of monoclonal antibodies recognizing GPI-anchored ADP-ribosyltransferase ART4, the carrier of the Dombrock blood group antigens. Cell Immunol. 236(1-2): 59-65.
  2. Friedrich M, et al. (2005) Analysis of the 3' UTR of the ART3 and ART4 gene by 3' inverse RACE-PCR. DNA Seq. 16(1): 53-7.
  3. Okazaki IJ, et al. (1998) Glycosylphosphatidylinositol-anchored and secretory isoforms of mono-ADP-ribosyltransferases. J Biol Chem. 273(37): 23617-20.

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