Murine RNase Inhibitor ELISA Kit _ 36707ES yeasen
$495.00
In stock
SKU
36707ES48
Product Description
Murine RNase inhibitor is a specific inhibitor of ribonuclease (RNase) found in the human placenta, which specifically inactivates RNase by binding to it non-covalently to form a complex that inactivates RNase. This kit uses the principle of double-antibody sandwich enzyme-linked immunosorbent assay (sandwich ELISA) to detect the residual Murine RNase Inhibitor in samples . First, add the Murine RNase Inhibitor standard and test sample to the Anti-Murine RNase Inhibitor coated microtiter strips (36707-A) then add the diluted biotin labeled Anti-Murine RNase Inhibitor antibod y (36707-C) and finally add Streptavidin-H RP (36707-D) to form an antibody + antigen + antibody-Biotin + SA-HRP complex. Subsequently, add TMB substra te (36707-H) i n to the complex to observe color reaction after washing the complex. TMB is converted into blue under the catalysis of HRP enzyme and finally converted into yellow in the presence of acid, and the shade of color is positively correlated with the amount of Murine RNase Inhibitor in the sample.
The detection range of this kit is 2~128 ng/mL; the lower detection limit is 1.5 ng/mL.
Features
- Highly sensitive:
- Detecting as little as
- 1.5
- ng/mL of
- Murine RNase Inhibitor
- residuals in in-process and final biological samples.
- Ensures accuracy:
- The
- Murine RNase Inhibitor
- residuals can be accurately detected with a recovery of
- 75
- %-1
- 25
- %.
- Specificity:
- Cooperate use with YEASEN
- Murine RNase Inhibitor
- and can accurately detect the residuals.
- Stable:
- The difference between lot-to-lot is low, the kit property is not impacted under 37℃ for
- 7
- days.
- Easy signal collection:
- Using Biotin-Streptavidin system, the signal can be stably enlarged.
Applications
- Residual
- Murine RNase Inhibitor
- test in biological products
- Residual Protein Detection in mRNA Vaccine and Therapeutic Production;
- Purification Validation in Nucleic Acid Synthesis and In Vitro Transcription (IVT)
Specifications
- Sensitivity
- 1.5 ng/mL (range 2~128 ng/mL)
- Assay Time
- <4 hours
- Assay Principle
- Two-site immunoenzymetric assay
- Signal Amplification
- Biotin-Streptavidin system
- Detection Wavelength
- 450 nm-630 nm
Components
- Components No.
- Name
- 36707ES48
- 36707ES96
- 36707-A
- Anti-Murine RNase Inhibitor coated microtiter strips
- 48 T
- 96 T
- 36707-B
- Standard: Murine RNase Inhibitor
- 1 vial
- 2 vial
- 36707-C
- Detection Antibody
- Biotin-conjugated Antibodies
- 60 μL
- 120 μL
- 36707-D
- Streptavidin-HRP
- 20 μL
- 40 μL
- 36707-E
- Dilution Buffer 1
- 25 mL
- 45 mL
- 36707-F
- Wash Buffer Concentrate (20×)
- 25 mL
- 50 mL
- 36707-G
- Dilution Buffer 2
- 15 mL
- 30 mL
- 36707-H
- TMB Substrate
- 8 mL
- 15 mL
- 36707-I
- Stop Solution
- 5 mL
- 10 mL
- 36707-J
- Plate Sealer
- 3 each
- 5 each
- The Standard (36707-B) is Lyophilized powder.
Storage
This product should be stored at 2°C ~8°C. Unopened product is valid for one year. * Upon receipt of the kit, please check whether all components are complete and immediately store them in corresponding condition.
Figures
Precision A) Repeatability Spiked sample Intra-Assay Duplicates Average Conc. (ng/mL) CV (%) S-64 ng/mL 10 62.538 2.2% S-32 ng/mL 10 33.477 6.2% S-4 ng/mL 10 3.851 8.2% B) Intermediate precision Spiked sample Inter-Assay Duplicates Average Conc. (ng/mL) CV (%) S-64 ng/mL 30 63.800 3.9% S-32 ng/mL 30 33.533 6.7% S-4 ng/mL 30 4.028 13.2% Table 1 . The precision of Murine RNase Inhibitor ELISA was assayed as the above scheme 10 duplicated were tested at 3 concentration s on the standard curve, and all the CV% were lower than 10 % ( Table 1 A). 3 lot of Salt Active UltraNuclease ELISA kits were tested and the CV% were lower than 1 5 % ( Table 1B ). Accuracy Three concentrations of Murine RNase Inhibitor standards, high, medium and low, were added to the mRNA purified samples in equal proportions, while the high value spiked samples were made multiplicative dilutions. Spiked recovery = measured value / (spiked standard x 50% + spiked specimen value x 50%)
Sample dilution ratio Measured value (ng/mL) Dilution linearity Sample spiking Measured value (ng/mL) Recovery rate S+Std1 71.493 S+Std1 71.493 110.3% 2× 43.571 121.9% S+Std3 20.569 122.2% 4× 22.174 101.8% S+Std5 5.516 114.2% 8× 13.132 118.5% S 1.661 16× 6.058 92.3% Table 2 Accuracy Validation The linear and spiked recoveries of Murine RNase Inhibitor in the doubly diluted samples, as well as the samples with different spiked concentrations, and in the unspiked samples, ranged from 80% to 125% (Table 2).
Safety Data Sheet
- 36707_MSDS_HB250226.pdf
Manuals
- 36707_Manual_Ver.EN20250226.pdf
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