Cynomolgus GDF15 Protein, His Tag yeasen
$175.00
In stock
SKU
94005ES25
Specifications
| Synonyms | NAG-1; MIC-1;GDF-15; PLAB |
| Source | Cynomolgus GDF15 Protein is expressed from E.coli with His tag at the N-Terminus. It contains Arg193-Val308.[Accession | G7PWZ3] |
| Source | The protein has a predicted MW of 14.15 kDa. The protein migrates to 15-18 kDa based on SDS-PAGE result. |
| Purity | > 95% as determined by SDS-PAGE |
| Formulation | Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 50mM HAc (pH 2.9). |
| Activity | ELISA Data: Immobilized Cynomolgus GDF15, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA .Immobilized Cynomolgus GDF15, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-GDF15 Antibody, hFc Tag with the EC50 of 6.1ng/ml determined by ELISA. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt. 2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution. 3 -6 months, -85~-65℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Notes
1.Please operate with lab coats and disposable gloves for your safety. 2.This product is for research use only.
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