Human B7-H3/CD276 Protein, hFc Tag yeasen
$130.00
In stock
SKU
93288ES25
Specifications
| Source | Recombinant Human B7-H3/CD276 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Leu29-Pro245.[Uniprot| Q5ZPR3-2] |
| Molecular Weight | The protein has a predicted MW of 50.1 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
| Purity | > 95% as determined by Tris-Bis PAGEand HPLC |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Activity | ELISA Data :Immobilized Human B7-H3, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-B7-H3 Antibody, hFc Tag with the EC50 of 42.2ng/ml determined by ELISA. |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt. 2-7 days, 2 ~8 °C under sterile conditions after reconstitution. 3 months, -25~-15℃ under sterile conditions after reconstitution. Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Notes
1.Please operate with lab coats and disposable gloves,for your safety. 2.This product is for research use only.
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