DNA polymerase I (10 U/μL) _ 12903ES yeasen
$45.00
In stock
SKU
12903ES60
Product Description
DNA Polymerase I, in the presence of template and primer (DNA or RNA), uses dNTP as a substrate to synthesize DNA complementary to the template in the 5'→3' direction. The molecular weight of this enzyme is about 109,000 Da, and it has double-strand-specific 5'→3' exonuclease activity and single-strand-specific 3'→5' exonuclease activity.
Applications
- With DNase I (Cat#10607) for nick translation reaction
- With RNase H (Cat#12906) to synthesize the second strand of cDNA
Specifications
| Concentration | 10 U/μL |
| Unit Definition | One unit is defined as the amount of enzyme required to degrade 1 µg of circular plasmid within a 10-minute period at 37°C in a 100 µl reaction system |
Components
- Components No.
- Name
- 12903ES60
- 12903ES76
- 12903ES92
- 12903-A
- DNA polymerase I (10 U/μL)
- 50 μL
- 250 μL
- 1 mL
- 12903-B
- 10×DNA polymerase I Reaction Buffer
- 1 mL
- 1×5 mL
- 4×5 mL
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 2 years.
Safety Data Sheet
- 12903_MSDS_Ver.EN20241210.pdf
Manuals
- 12903_Manual_Ver.EN20250729.pdf
Related Blog
- DNase I Applications
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