Hieff™ Bacterial RNA Kit _ 19301ES yeasen
$210.00
In stock
SKU
19301ES50
Product Description
This Kit is designed for rapid and efficient RNA extraction from Gram-positive and Gram-negative bacteria.Utilizing a proprietary silica- membrane column technology combined with optimized buffers, this kit effectively removes contaminants such as proteins, metabolites, and genomic DNA. The purified RNA is suitable for downstream applications including RT-PCR, RT-qPCR, in vitro transcription, and molecular cloning.
Specifications
- Cat.N
- o
- 19301
- ES50
- Size
- 50 T
Components
- Category
- Components No.
- Name
- 19301
- ES50
- Part I
- 19301
- -A
- Lysozyme
- 20
- mg
- Part II
- 19301
- -B
- DNA-Removing Column B4
- 50
- units
- 19301
- -C
- Hieff™
- RNA
- Column
- B
- 4
- 50
- units
- 19301
- -D
- 2
- mL
- Collection
- Tube
- B
- 4
- 100
- units
- 19301
- -E
- Lysis Buffer(
- LB
- Buffer
- B
- 4
- )
- 25
- mL
- 19301
- -F
- PL
- Buffer
- B
- 4
- 40
- mL
- 19301
- -G
- Wash Buffer
- B4
- 13 mL(add 52 mL ethanol
- before
- use
- )
- 19301-H
- TE
- Buffer
- pH
- 8.0
- 6
- mL
- 19301-I
- RNase
- free
- H
- 2
- O
- 5
- mL
Features
- Ø
- Wide applicability
- can be used for bacterial cultures such as Escherichia coli, Pseudomonas aeruginosa, and Gram-positive bacteria.
- Ø
- Quick and easy
- : the operation can be completed within 40 minutes.
- Ø
- High RNA purity
- : DNase I on-column digestion effectively removes gDNA.
- Ø
- Safe and environmentally friendly
- : no need to use organic matter such as phenol and chloroform for extraction.
Applications
- Bacterial RNA extraction；RNA extraction from Gram-positive bacteria；Gram-negative bacteria, etc.
Shipping and Storage
Part I
Components
- should be stored
- at 4~8℃ for 2 years.
- Part II components
- should be stored at
- room temperature
- for
- 2
- years
Figures
Suppliers Concentration OD260/OD280 OD260/OD230 Yeasen-19031ES 159.40/193.18 1.895/1.925 2.177/2.101 Supplier T* 182.01/179.31 1.855/1.922 0.728/1.206 Table 1. E. coli extract product co ncentration
Figure 1. Electrophoresis of E. coli RNA extraction products Take 4 portions of 2 mL of E. coli suspension, use 19301 and similar products from supplier T brand to extract total RNA, and elute with 50 ul RNase-free H2O. Then take 1 ul of the eluate to determine the concentration. Take 5 ul of the eluate to identify the RNA integrity and band brightness. The results show that the 19301 extraction product has a high yield and good RNA integrity.
Safety Data Sheet
- 19301ES-MSDS-HB250516.PDF
Manuals
- 19301_Manual_Ver. EN20250825_EN.pdf
Citations & References
- [1] Ma X, Hou M, Liu C, Li J, Ba Q, Wang H. Cadmium accelerates bacterial oleic acid production to promote fat accumulation in Caenorhabditis elegans. J Hazard Mater. 2022;421:126723. doi:10.1016/j.jhazmat.2021.126723(IF:10.588)
- [2] Wang Y, Hu W, Deng Z, He X. Rapid identification of magnesium ascorbyl phosphate utilizing phosphatase through a chromogenic change-coupled activity assay. Appl Microbiol Biotechnol. 2021;105(7):2901-2909. doi:10.1007/s00253-021-11229-7(IF:4.813)
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