Mouse Lipopolysaccharide-binding protein(LBP) ELISA kit CSB-EL012775MO CUSABIO
$50.00
In stock
SKU
CSB-EL012775MO
Product Details
| Target Name | lipopolysaccharide binding protein |
| Uniprot No. | Q61805 |
| Species | Mus musculus (Mouse) |
| Storage | Store at 2-8°C. Please refer to protocol. |
| Sensitivity | 3.12 ng/mL |
| Troubleshooting and FAQs | ELISA kit FAQs |
| Lead Time | 3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx. |
| Associated names | LbpLipopolysaccharide-binding protein ELISA Kit; LBP ELISA Kit |
| Abbreviation | LBP |
| Sample Types | serum, plasma, tissue homogenates |
| Detection Range | 12.5 ng/mL-800 ng/mL |
| Assay Time | 1-5h |
| Sample Loading Volume | 50-100ul |
| Detection Wavelength | 450 nm |
| Research Area | Immunology |
| Assay Principle | quantitative |
| Measurement | Sandwich |
| Precision | headers: Intra-assay Precision (Precision within an assay): CV%<8%; rows: Intra-assay Precision (Precision within an assay): CV%<8%: Intra-assay Precision (Precision within an assay): CV%<8%; 1: Three samples of known concentration were tested twenty times on one plate to assess.; Intra-assay Precision (Precision within an assay): CV%<8%: Inter-assay Precision (Precision between assays): CV%<10%; 1: Three samples of known concentration were tested in twenty assays to assess.; |
| Linearity | headers: To assess the linearity of the assay, samples were spiked with high concentrations of mouse LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; rows: To assess the linearity of the assay, samples were spiked with high concentrations of mouse LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.: To assess the linearity of the assay, samples were spiked with high concentrations of mouse LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.; 2: Sample; 3: Serum(n=4); 1: 1:1; 2: Average %; 3: 87; 1: Range %; 2: 82-92; 1: 1:2; 2: Average %; 3: 102; 1: Range %; 2: 97-107; 1: 1:4; 2: Average %; 3: 98; 1: Range %; 2: 94-101; 1: 1:8; 2: Average %; 3: 93; 1: Range %; 2: 90-96 |
| Recovery | headers: The recovery of mouse LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; rows: The recovery of mouse LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.: The recovery of mouse LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.; 1: Sample Type; 2: Average % Recovery; 3: Range; 1: Serum (n=5); 2: 89; 3: 85-93; 1: EDTA plasma (n=4); 2: 96; 3: 91-102; ; |
| Typical Data | headers: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; rows: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.: These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.; 1: ng/ml OD1 OD2 Average Corrected 800 2.922 2.974 2.948 2.803 400 2.166 2.159 2.163 2.018 200 1.447 1.448 1.448 1.303 100 0.913 0.925 0.919 0.774 50 0.524 0.565 0.545 0.400 25 0.335 0.312 0.324 0.179 12.5 0.253 0.258 0.256 0.111 0 0.140 0.150 0.145; 2: ng/ml; 3: OD1; 4: OD2; 5: Average; 6: Corrected; 8: 800; 9: 2.922; 10: 2.974; 11: 2.948; 12: 2.803; 14: 400; 15: 2.166; 16: 2.159; 17: 2.163; 18: 2.018; 20: 200; 21: 1.447; 22: 1.448; 23: 1.448; 24: 1.303; 26: 100; 27: 0.913; 28: 0.925; 29: 0.919; 30: 0.774; 32: 50; 33: 0.524; 34: 0.565; 35: 0.545; 36: 0.400; 38: 25; 39: 0.335; 40: 0.312; 41: 0.324; 42: 0.179; 44: 12.5; 45: 0.253; 46: 0.258; 47: 0.256; 48: 0.111; 50: 0; 51: 0.140; 52: 0.150; 53: 0.145; 1: ng/ml; 2: OD1; 3: OD2; 4: Average; 5: Corrected; 1: 800; 2: 2.922; 3: 2.974; 4: 2.948; 5: 2.803; 1: 400; 2: 2.166; 3: 2.159; 4: 2.163; 5: 2.018; 1: 200; 2: 1.447; 3: 1.448; 4: 1.448; 5: 1.303; 1: 100; 2: 0.913; 3: 0.925; 4: 0.919; 5: 0.774; 1: 50; 2: 0.524; 3: 0.565; 4: 0.545; 5: 0.400; 1: 25; 2: 0.335; 3: 0.312; 4: 0.324; 5: 0.179; 1: 12.5; 2: 0.253; 3: 0.258; 4: 0.256; 5: 0.111; 1: 0; 2: 0.140; 3: 0.150; 4: 0.145 |
| ELISA Data Analysis | ELISA Standard Curve & Curve Expert software |
| Shelf Life | 6 months |
| Description | Lipopolysaccharide-binding protein (LBP) is an acute-phase protein that functions in the innate immune response to bacterial infections. This glycoprotein binds to lipopolysaccharide from gram-negative bacteria and transfers it to CD14 and Toll-like receptor 4, which initiates inflammatory cascades. LBP levels rise significantly during sepsis and other inflammatory conditions. This makes it a valuable biomarker for monitoring immune activation and bacterial infection severity in research applications. The Mouse Lipopolysaccharide-binding protein ELISA kit (CSB-EL012775MO) uses a quantitative sandwich measurement principle for detecting LBP in Mus musculus samples. This assay works with serum, plasma, and tissue homogenates with a detection range of 12.5 ng/mL to 800 ng/mL and sensitivity of 3.12 ng/mL. The protocol requires 50-100 μL sample volume and takes 1-5 hours to complete, with detection performed at 450 nm wavelength. Application Examples Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section. This ELISA kit has been used in research studies to measure lipopolysaccharide-binding protein levels in plasma samples from mouse models. Applications include inflammatory response research and studies examining how various interventions affect systemic biomarkers. • Inflammatory biomarker profiling - Measurement of lipopolysaccharide-binding protein alongside other inflammatory markers including cytokines in plasma samples to assess systemic inflammatory responses • Endocrine and metabolic research - Analysis of lipopolysaccharide-binding protein levels in studies investigating hormonal parameters and metabolic function in mouse models • Intervention studies - Quantification of plasma lipopolysaccharide-binding protein to evaluate how experimental treatments or exposures affect inflammatory biomarker profiles |
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