Recombinant B.subtilis sfp (C-6His)(Cat. No.:CH20) novoprotein
$119.00
In stock
SKU
CH20
10μg: $119
50μg: $356
500μg: $1663
1mg: $2500
Description
Recombinant B.subtilis 4'-phosphopantetheinyl Transferase Sfp/Sfp is produced by our E.coli expression system and the target gene encoding Met1-Leu224 is expressed with a 6His tag at the C-terminus.
Product Details
| Item | Details |
| Known As | 4'-phosphopantetheinyl transferase sfp; Surfactin synthase-activating enzyme; sfp; lpa-8 |
| Mol Mass | 27.2 KDa |
| APMol Mass | 28 KDa, reducing conditions |
| Formulation Description | Supplied as a 0.2 μm filtered solution of 20mM PB, 100mM NaCl, pH 7.0. |
| Endotoxin | < 1 EU/µg as determined by LAL test. |
| Storage | Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles. |
| Shipping | The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below. |
Background
The Bacillus subtilis enzyme Sfp, required for production of the lipoheptapeptide antibiotic surfactin, posttranslationally phosphopantetheinylates a serine residue in each of the seven peptidyl carrier protein domains of the first three subunits (SrfABC) of surfactin synthetase to yield docking sites for amino acid loading and peptide bond formation.
title: Modular catalytic activity of nonribosomal peptide synthetases depends on the dynamic interaction between adenylation and condensation domains
authors: Ye-Jun Peng, Yuxing Chen, Cong-Zhao Zhou, Wei Miao, Yong-Liang Jiang, Xiaoli Zeng, Cheng-Cai Zhang
date: 2024/02/09
journal: STRUCTURE
MDSD
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