LbaCas12a Nuclease(Cat. No.:E373-YH01) novoprotein
$214.00
In stock
SKU
E373-YH01
50ug: $214
500ug: $667
Description
Cas12a (previously known as Cpf1) belongs to the Class 2 Type V CRISPR/Cas system. Different from Cas9 protein, Cas12a does not require tracrRNA, but only a single crRNA guide consisting of 41-44nt nucleotides. In contrast to Cas9, which requires a G-rich PAM, the Cas12a-crRNA complex recognizes a T-rich (5'-TTTN-3') protospacer adjacent motif (PAM) and results in a staggered DNA double-strand break (DSB). After the specific cleavage, Cas12a can also activate collateral cleavage activity towards adjacent non-specific ssDNA sequences. Hence, Cas12a nuclease is a good alternative for Cas9 in certain target DNA editing, and provides a novel strategy for DNA detection.
LbaCas12a Nuclease is an RNA-guided DNA endonuclease from Lachnospiraceae bacterium, with a nuclear localization signal (NLS) added to both ends of the protein.
Usage
Genome Editing, DNA Detection, CRISPR Diagnostics
Product Details
| Item | Details |
| Features | Ribonucleoprotein (RNP)-mediated CRISPR genome editing is more effective and avoids the problems associated with using plasmids. Significantly reduce off-target effects. |
Quality Control
After multiple column purifications, only a clear single target band was visible in SDS-PAGE gel detection; qPCR method detection showed no residual E. coli DNA and no RNase, endonuclease or exonuclease contamination. Purity up to 90%, endotoxin < 100EU/mg.
Product Details
| Item | Details |
| Storage | -20℃. |
Components
| Component | E373-YH01-01A (Size：50μg) | E373-YH01-01B (Size：500μg) |
| LbaCas12a Nuclease (2.5μg/μl) | 20μl | 200μl |
| 10×Cas12a-dsDNA Buffer I | 500μl | 1ml×5 |
| 10×Cas12a-ssDNA Buffer II | 500μl | 1ml×5 |
title: Specific and visual identification of the culinary-medicinal mushroom Pleurotus tuber-regium by ITS-based CRISPR/Cas12a assay
authors: Caiyun Long, Linhuizi Xiang, Dongmei Liu, Ting Hu, Gong Chen, Zhilong Wang, Binrong Ke, Xueyan Sun, Xihong Zhao
date: 2026/07/21
journal: MICROCHEMICAL JOURNAL
title: Dual-locking dCas13a/crRNA and duplex-specific nuclease cascade for ultrasensitive and accurate detection of ovarian cancer-related circular RNA
authors: Dongmei Zhou, Yinan Jiang, Yan Liu, Peili Liang, Yuping Zhang, Haijiao Zou, Jianqi Li
date: 2026/07/17
journal: MICROCHEMICAL JOURNAL
title: CRISPR/enzyme cascade-driven transistor overcomes Debye screening for culture-free detection of resistant bacteria
authors: Jiahao Li, Min Jia, Han Liu, Weini Xin, Zhi-Yong Zhang, Chen-Zhong Li, Guo-Jun Zhang, Cheng Jiang
date: 2026/07/12
journal: CHEMICAL ENGINEERING JOURNAL
title: Integrated CRISPR-Cas13a/Cas12a system for multiplexed detection of glaucoma-associated microRNAs
authors: Xia Cheng, Wenchen Zhao, Xinyi Xia, Shiqi Shen, Mengqing Li, Yuqiu Li, Daixi Chen, Tianwei Qian, Kun Yin, Ping Yu, Xiaolan Bian
date: 2026/05/30
journal: MICROCHEMICAL JOURNAL
title: Aptamer-Mediated Protein Corona In Situ Multiplex Microfluidic Detection for Cancer Diagnosis
authors: Weizhao Yao, Yaru Li, Yuling Du, Rongzi Gao, Junhao Tan, Jinlong Fan, Yuan Liu
date: 2026/2/25
journal: ANALYTICAL CHEMISTRY
title: Sensitive detection of piRNAs using PAM-free CRISPR-Cas12a system integrated with primer extension amplification
authors: Xiang Chen, Yan Yu, Zhigang Liang, Lulu Qin, Guanhong Xu, Fangdi Wei, Jing Yang, Qin Hu, Yao Cen
date: 2026/01/13
journal: SENSORS AND ACTUATORS B-CHEMICAL
title: A Relay CRISPR/Cas12a System for Extraction-Free and Ultrasensitive Detection of Staphylococcus aureus in Complex Food Samples
authors: Kun Wang, Wanli Wang, Yufeng Hu, Xiaoyang Li, Haoyu Yu, Xin Xia, Tianhan Kai, Mei Wen
date: 2026/01/07
journal: ANALYTICAL CHEMISTRY
title: Identification of ATG7 as a protein ATG8ylation substrate
authors: Huazhong Xie, Jiahui Long, Yijun Huang, Yao Wang, Peiqing Liu, Min Li
date: 2025/11/03
journal: Cell Reports
title: Novel electrochemiluminescence resonance energy transfer biosensor driven by CRISPR-Cas12a system for ctDNA detection
authors: Xiaocui Huang, Alian Wang, Zhenyu Lin, Yunpeng Xu, Jianping Zheng
date: 2025/10/03
journal: BIOSENSORS & BIOELECTRONICS
title: A rapid CRISPR-Cas12a/T7EI integrated workflow for high-throughput screening of homozygous mutant cell lines
authors: Guohui Xiao, Hongyu Shi, Qiao Lin, Siqi Li, Junyan He, Guoliang Zhang
date: 2025/09/21
journal: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
title: FEN1-guided autocatalytic DNA circuits integrated with CRISPR/Cas12a for ultrasensitive detection of a bacterial pathogen
authors: Luhu Yu, Xiaoyu Li, Yueming Huang
date: 2025/09/19
journal: MICROCHEMICAL JOURNAL
title: Trans-cleavage activity of Cas12a effectors can be unleashed by both double-stranded DNA and single-stranded RNA targeting in absence of PAM
authors: Guohui Xiao, Hongyu Shi, Meixia Liu, Min Huang, Siqi Li, Xuefeng Zhou, Hengfei Li, Guoliang Zhang
date: 2025/04/11
journal: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
title: Split crRNA Precisely Assisted Cas12a Expansion Strategy for Simultaneous, Discriminative, and Low-Threshold Determination of Two miRNAs Associated with Multiple Sclerosis
authors: Xinyi Xia, Zhigang Liang, Guanhong Xu, Fangdi Wei, Jing Yang, Xiaolei Zhu, Chenglin Zhou, Jun Ye, Qin Hu, Zheng Zhao, Ben Zhong Tang, Yao Cen
date: 2025/02/03
journal: ANALYTICAL CHEMISTRY
title: Development of an on-site real-time dual detection method for norovirus and rotavirus using RPA-CRISPR/Cas12,13
authors: Xiangyun Le, Jing Jiang, Yi Hong, Juping Shi, Xuejie Liu, Junxin Xue, Xiang Wang
date: 2024/10/16
journal: FOOD CONTROL
title: Aggregation-Induced Electrochemiluminescence of Ir(ppy)3-Functionalized ZIF-8 for Microcystin-LR Detection via the trans-Cleavage Activity of CRISPR-Cas12a
authors: Binnan Shi, Yue Jia, Dehao Jia, Tian Tian, Jingui Chen, Xiang Ren, Jianping Lei, Hongying Jia, Huan Wang, Qin Wei
date: 2024/09/05
journal: ANALYTICAL CHEMISTRY
title: An electrochemiluminescent imaging strategy based on CRISPR/Cas12a for ultrasensitive detection of nucleic acid
authors: Sijian Luo, Jie Wu, Min Zhong, Jun Sun, Hang Ao, Xu Cao, Jinbo Liu, Huangxian Ju
date: 2024/07/31
journal: ANALYTICA CHIMICA ACTA
title: CoHIT: a one-pot ultrasensitive ERA-CRISPR system for detecting multiple same-site indels
authors: Liu Yin, Liu Xinyi, Wei Dongyi, Dang Lu, Xu Xiaoran, Huang Shisheng, Li Liwen, Wu Sanyun, Wu Jinxian, Liu Xiaoyan, Sun Wenjun, Tao Wanyu, Wei Yongchang, Huang Xingxu, Li Kui, Wang Xinjie, Zhou Fuling
date: 2024/06/12
journal: Nature Communications
title: Self-assembled bifunctional nanoflower-enabled CRISPR/Cas biosensing platform for dual-readout detection of Salmonella enterica
authors: Manyan Qiu, Zhiyu Yuan, Nan Li, Xinyan Yang, Xianlong Zhang, Yujun Jiang, Qianyu Zhao, Chaoxin Man
date: 2024/04/16
journal: JOURNAL OF HAZARDOUS MATERIALS
title: Rapid Nucleic Acid Detection of Listeria monocytogenes Based on RAA-CRISPR Cas12a System
authors: Yujuan Yang, Xiangxiang Kong, Jielin Yang, Junxin Xue, Bing Niu, Qin Chen
date: 2024/03/20
journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
title: CRISPR/Cas12a-Responsive Hydrogels for Conjugation-Free and Universal Indicator Release in Colorimetric Detection
authors: Rujian Zhao, Yidan Tang, Defeng Song, Meng Liu, Bingling Li
date: 2023/12/06
journal: ANALYTICAL CHEMISTRY
title: Development of a CRISPR/Cas12a-recombinase polymerase amplification assay for visual and highly specific identification of the Congo Basin and West African strains of mpox virus
authors: Xinggui Yang, Xiaoyan Zeng, Xu Chen, Junfei Huang, Xiaoyu Wei, Xia Ying, Qinqin Tan, Yi Wang, Shijun Li
date: 2023
journal: JOURNAL OF MEDICAL VIROLOGY
title: Red fluorescence-emitting copper nanoparticles-assisted label-free bioprobe coupled with CRISPR/Cas12a for sensitive detection of T4 polynucleotide kinase
authors: Zhigang Liang, Xinyang Li, Chen Chen, Feifei Xu, Guanhong Xu, Fangdi Wei, Jing Yang, Qin Hu, Jianjun Zou, Yao Cen
date: 2023
journal: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
title: Development of Recombinase-Aided Amplification (RAA)-Exo-Probe and RAA-CRISPR/Cas12a Assays for Rapid Detection of Campylobacter jejuni in Food Samples
authors: Shuai Zhi, Jinling Shen, Xingang Li, Yuan Jiang, Junxin Xue, Taisong Fang, Jin Xu, Xuan Wang, Yuhao Cao, Danting Yang, Zhiyuan Yao, Daniel Yu
date: 2022
journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
title: CRISPR/Cas12a-based fluorescence biosensor for detection of exosomal miR-21 derived from lung cancer
authors: Zhe Lu, Wei Ni, Nian Liu, Dan Jin, Tingxian Li, Kun Li, Yuling Zhang, Qunfeng Yao, Guo-Jun Zhang
date: 2022
journal: MICROCHEMICAL JOURNAL
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