ChromoTek V5-Trap® Magnetic Particles M-270 v5td proteintech
$352.00
In stock
SKU
v5td
| Synonyms | Magnetic Particles M-270, V5-Trap Magnetic Particles M-270, V5 tag, V5-tag, V5-Trap®, V5-Trap® Magnetic Particles M-270, v5td, v5td-10, v5td-100, v5td-20, v5td-200, v5td-400 |
| Host | Alpaca |
| Formulation | United States |
| Applications | IP, CoIP, ChIP, RIP, Protein purification |
| Host / Isotype | IP, CoIP, ChIP, RIP |
| Specificity/Target | V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein. |
| Binding capacity | 0.62-1.25 μg of recombinant V5-tagged protein (~30 kDa) per 25 μL bead slurry |
| Conjugate | Magnetic Particles M-270, size: 2.8 µm high throughput-compatible |
| Elution buffer | V5-peptide SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 2 % Nonidet P40 Substitute, 2 % Triton X-100, 150 mM NaCl, 10 mM TCEP, 10 mM DTT, 50 mM Urea |
| Type | Nanobody |
| Class | Recombinant |
| Affinity (K D ) | Dissociation constant K D of 40 nM |
| Compatibility with mass spectrometry | The V5-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2892994 |
| Storage Buffer | PBS with 0.09% sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
| IP | iScience Enzymatically controlled release of proteins and peptides: A promising, alternative secretion approach. Authors - Daniel Ivanusic View Article |
| RIP | Insect Sci An ovary-enriched piRNA controls ovarian development via Gadd45a-JNK-mediated nurse-cell apoptosis in the arbovirus vector Aedes albopictus. Authors - Lu Yang View Article |
| FH Magdalena (Verified Customer) (02-12-2025) | The pulldown of V5-tagged protein worked very well with this product. The yield was a lot better than with the magnetic agarose. The only issue was when eluting with LSB (as stated in the protocol), a precipitate or broken resin formed, which was not magnetic anymore and had to be spun down before gel loading. Applications: Immunoprecipitation |
| FH Karel (Verified Customer) (11-20-2024) | The purity was excellent as we used the sample of masss pecrometry analysis. Applications: Protein purification Cell Tissue Type: yeast |
| FH Karel (Verified Customer) (03-14-2024) | reproducible purification without background. The purified proteins we want to use for mass spec analysis Applications: ChIP,Protein purification |
| FH Tsimafei (Verified Customer) (12-04-2022) | IP was performed using cells expressing V5-tagged mCherry. 10µl of beads were used for 0.5mg of total protein. Excellent performance! Applications: Immunoprecipitation Cell Tissue Type: mESCs |
Description
Immunoprecipitation of V5-tagged proteins and their interacting factors with anti-V5 Nanobody conjugated to magnetic particles. V5-Trap Magnetic Particles M-270 is recommended when very large proteins/complexes are investigated and magnetic separation is needed for IP. • No heavy & light antibody chains in downstream applications • Very low background • Effective elution with V5-peptide • Stable under harsh washing conditions • Efficient immunoprecipitation of V5-tag fusion proteins
Publications
- On the possibility of yet a third kinetochore system in the protist phylum Euglenozoa
Journal: mBio | Authors: Corinna Benz | Species: IP | Application: mBio On the possibility of yet a third kinetochore system in the protist phylum Euglenozoa Authors - Corinna Benz View Article - Functional differentiation of Sec13 paralogues in the euglenozoan protists
Journal: Open Biol | Authors: Drahomíra Faktorová | Species: IP | Application: Open Biol Functional differentiation of Sec13 paralogues in the euglenozoan protists Authors - Drahomíra Faktorová View Article - An ovary-enriched piRNA controls ovarian development via Gadd45a-JNK-mediated nurse-cell apoptosis in the arbovirus vector Aedes albopictus.
Journal: Insect Sci | Authors: Lu Yang | Species: RIP | Application: Insect Sci An ovary-enriched piRNA controls ovarian development via Gadd45a-JNK-mediated nurse-cell apoptosis in the arbovirus vector Aedes albopictus. Authors - Lu Yang View Article - Enzymatically controlled release of proteins and peptides: A promising, alternative secretion approach.
Journal: iScience | Authors: Daniel Ivanusic | Species: IP | Application: iScience Enzymatically controlled release of proteins and peptides: A promising, alternative secretion approach. Authors - Daniel Ivanusic View Article - N-Glycosylation of mGluR6 Modulates Receptor Cell-Surface Transport, G-Protein Coupling, and Interactions With Synaptic Adhesion Molecules.
Journal: J Mol Neurosci | Authors: Takumi Akagi | Application: J Mol Neurosci N-Glycosylation of mGluR6 Modulates Receptor Cell-Surface Transport, G-Protein Coupling, and Interactions With Synaptic Adhesion Molecules. Authors - Takumi Akagi View Article - Proximity Labeling Reveals How Lrp2 Interacts with the Endocytic Machine.
Journal: J Proteome Res | Authors: Tian H. Shen | Application: J Proteome Res Proximity Labeling Reveals How Lrp2 Interacts with the Endocytic Machine. Authors - Tian H. Shen View Article - The SPARK complex forms the molecular basis of vertebrate fertilization.
Journal: bioRxiv | Authors: Victoria E. Deneke | Application: IP
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