Human E-selectin / CD62E ELISA Kit KE00611 proteintech
$299.00
In stock
SKU
KE00611
| Product name | Human E-selectin / CD62E ELISA Kit |
| Synonyms | CD62E, E-selectin, CD62 antigen-like family member E, E selectin, ELAM |
| Reactivity | Human |
| Host / Isotype | United States |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma |
| Assay type | Sandwich |
| Sensitivity | 2.8 pg/mL |
| Range | 15.6-1000 pg/mL |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6401 |
| Sample Type | Average Range |
| Human serum | 106% 103%-112% |
| Sample | n mean ( pg/mL ) SD CV% |
| 1 | 16 452.7 16.1 3.6 |
| 2 | 16 116.5 4.1 3.5 |
| 3 | 16 59.1 5.1 8.6 |
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
E-selectin (Endothelial Leukocyte Adhesion Molecule-1, also known as CD62E) is a critical adhesion molecule expressed on endothelial cells. It plays a crucial role in the initial steps of leukocyte recruitment to sites of inflammation, facilitating the rolling and adhesion of leukocytes to the endothelium. This process is essential for the immune response to infection and injury. E-selectin is upregulated in response to inflammatory cytokines such as IL-1, TNF-α, and bacterial lipopolysaccharide (LPS), and it interacts with various glycan ligands on leukocytes, including PSGL-1, ESL-1, CD44, and CD43. Properties Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. Synonyms CD62E, E-selectin, CD62 antigen-like family member E, E selectin, ELAM FAQ 1、I have limited sample diluent. How should I handle samples requiring high dilution factors? Answer: We recommend performing a serial dilution. For example, if human serum requires a 1,000,000-fold dilution, you can use a three-step 100× dilution method as follows: Step 1: Take 2 uL of human serum and add it to 198 uL of sample diluent, mix thoroughly to obtain a 100-fold diluted sample. Step 2: Take 2 uL of the 100-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 10,000-fold diluted sample. Step 3: Take 2 uL of the 10,000-fold diluted sample and add it to 198 uL of sample diluent, mix thoroughly to obtain a 1,000,000-fold diluted sample. If further dilution is required, additional serial steps can be used. We do not recommend using any other solutions or water for dilution, except for the sample diluent we suggest. If your sample diluent is still insufficient, please contact our customer service for more sample diluent.
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