| Catalog No. | Size | Price (USD) |
| 90011-MM02-50 | 50 µL | $147.96 |
| 90011-MM02-100 | 100 µL | $251.64 |
| 90011-MM02-200 | 200 µL | $355.32 |
General Information
| Product name | Anti-IL-18 Antibody, Mouse Monoclonal |
| Validated applications | ELISA (FAQ Protocol) |
| Species reactivity | Reacts with: Rhesus |
| Specificity | Rhesus IL-18 No cross-reactivity in ELISA with E.coli cell lysate |
| Immunogen | Recombinant Rhesus IL18 / Interleukin-18 Protein (Catalog#90011-C07E) |
| Preparation | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rhesus IL18 / Interleukin-18(Catalog #90011-C07E; NP_001028006.1; Ala 2-Asp 193). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Source | Monoclonal Mouse IgG2a Clone #5G10E10 |
| Purification | Protein A |
| Formulation | 0.2 μm filtered solution in PBS |
| Conjugate | Unconjugated |
| Form | Liquid |
| Shipping | This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
Background Information
| Full Name | interleukin 18 |
| Description | Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1-like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cell -, IL-4- and STAT6-dependent fashion. IL-18 and T cell receptor-mediated stimulation could induce naive CD4+ T cells to develop into IL-4-producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in the regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-α and IL-1β, suggesting that monokine expression can feedback to promote Th1 cell development in the synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA. |
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| References |
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title: Safranal exerts a neuroprotective effect on Parkinson’s disease with suppression of NLRP3 inflammation activation
authors: Wenping Yang; Yongyue Wei; ...; Haixia Ding
journal: Molecular Biology Reports
date: 2024-04-29
pmid: 38683404
doi: 10.1007/s11033-024-09537-y
pmcid: 11059006
cited_by_count: 2
techniques: Expressing; Incubation; Membrane; Staining; Western Blot
bioz_score: 92
