Human ASGR1 ELISA Kit sinobiological KIT10773

$714.00
In stock
SKU
KIT10773
Catalog No.SizePrice (USD)
KIT10773-11 Kit (96 Tests)$740.55

General Information

Product nameHuman ASGR1 ELISA Kit
SpecificityThis assay recognizes both recombinant and natural Human ASGR1.
ConjugateHRP
ShippingThis ELISA Kit is shipped at ambient temperature.
StorageUnopened Kit: Store at 2 - 8℃ Opened/Reconstituted Reagents: Please refer to CoA
Assay typeSolid Phase Sandwich ELISA (quantitative)
Sample typeSerum,Cell supernatant
Sensitivity11.57 pg/mL
Assay range39.06-2500 pg/mL
Materials provided1. 96 well microplate coated with Capture Antibody 2. Detection Antibody conjugated to HRP 3. Standards 4. Wash Buffer Concentrate 5. Dilution Buffer Concentrate 6. Color Reagent A 7. Color Reagent B 8. Stop Solution
Product overviewThis Human ASGR1 ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of Human ASGR1 protein in Serum,Cell supernatant . It contains recombinant Human ASGR1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
InterferencePreparations of human CANX, NLK and APP at 40 ng/mL in a mid-range recombinant human ASGR1 control were assayed for interference. No significant interference was observed.

Synonyms: ASGPR ELISA Kit, Human; ASGPR1 ELISA Kit, Human; CLEC4H1 ELISA Kit, Human; HL-1 ELISA Kit, Human

Image / Experimental Information

Image 1 DescriptionThis standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Image 1 Alt TextHuman ASGPR1 ELISA Kit Standard Curve 70
Image 1 URLSource image
Image 2 DescriptionThis standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Image 2 Alt TextHuman ASGPR1 ELISA Kit Specificity 253
Image 2 URLSource image

Background Information

Full Nameasialoglycoprotein receptor 1
DescriptionThe asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development.
References
  1. Yang J, et al. (2006) Antisense oligonucleotides targeted against asialoglycoprotein receptor 1 block human hepatitis B virus replication. J Viral Hepat. 13(3): 158-65.
  2. Li Y, et al. (2008) Targeted delivery of macromolecular drugs: asialoglycoprotein receptor (ASGPR) expression by selected hepatoma cell lines used in antiviral drug development. Curr Drug Deliv. 5(4): 299-302.

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