Human Lymphotoxin alpha/TNF beta ELISA Kit sinobiological KIT10270

$400.00
In stock
SKU
KIT10270
Catalog No.SizePrice (USD)
KIT10270-11 Kit (96 Tests)$414.71

General Information

Product nameHuman Lymphotoxin alpha/TNF beta ELISA Kit
SpecificityThis assay recognizes both recombinant and natural Human TNFβ.
ConjugateHRP
ShippingThis ELISA Kit is shipped at ambient temperature.
StorageUnopened Kit: Store at 2 - 8℃ Opened/Reconstituted Reagents: Please refer to CoA
Assay typeSolid Phase Sandwich ELISA (quantitative)
Sample typeCell supernatant
Sensitivity0.9 pg/mL
Assay range7.8-500 pg/mL
Materials provided1. 96 well microplate coated with Capture Antibody 2. Detection Antibody conjugated to HRP 3. Standards 4. Wash Buffer Concentrate 5. Dilution Buffer Concentrate 6. Color Reagent A 7. Color Reagent B 8. Stop Solution
Product overviewThis Human Lymphotoxin alpha/TNF beta ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Lymphotoxin alpha/TNF beta protein in Cell supernatant . It contains recombinant Human Lymphotoxin alpha/TNF beta, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
InterferencePreparations of the following factors at 50 ng/mL in a mid-range recombinant Human TNFβ control were assayed for interference. No significant interference was observed.

Synonyms: LT ELISA Kit, Human; TNFB ELISA Kit, Human; TNFSF1 ELISA Kit, Human; TNLG1E ELISA Kit, Human

Image / Experimental Information

Image 1 DescriptionThis standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Image 1 Alt TextHuman TNF-beta/TNFSF1/Lymphotoxin alpha ELISA Kit Standard Curve 17207
Image 1 URLSource image
Image 2 DescriptionThis standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
Image 2 Alt TextHuman TNF-beta/TNFSF1/Lymphotoxin alpha ELISA Kit Specificity 17221
Image 2 URLSource image

Background Information

Full Namelymphotoxin alpha
DescriptionLymphotoxin-alpha, also known as LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA TNFSF1, and TNFB, is a secreted protein that belongs to the tumor necrosis factor family. TNF-beta/TNFSF1/Lymphotoxin alpha is highly inducible, secreted, and exists as a homotrimeric molecule. It is a cytokine that in its homotrimeric form binds to TNFRSF1A / TNFR1, TNFRSF1B / TNFBR, and TNFRSF14 / HVEM. In its heterotrimeric form with LTB, TNF-beta/TNFSF1/Lymphotoxin alpha binds to TNFRSF3 / LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells. TNF-beta/TNFSF1/Lymphotoxin alpha forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. It mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. TNF-beta/TNFSF1/Lymphotoxin alpha is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in TNF-beta/TNFSF1/Lymphotoxin alpha are a cause of susceptibility psoriatic arthritis which is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy.
Research Areas
  • Neuroinflammation
References
  1. Messer G, et al. (1991) Polymorphic structure of the tumor necrosis factor (TNF) locus: an NcoI polymorphism in the first intron of the human TNF-beta gene correlates with a variant amino acid in position 26 and a reduced level of TNF-beta production. J Exp Med. 173(1): 209-19.
  2. Banner DW, et al. (1993) Crystal structure of the soluble human 55 kd TNF receptor-human TNF beta complex: implications for TNF receptor activation. Cell. 73(3): 431-45.
  3. Picarella DE, et al. (1993) Transgenic tumor necrosis factor (TNF)-alpha production in pancreatic islets leads to insulitis, not diabetes. Distinct patterns of inflammation in TNF-alpha and TNF-beta transgenic mice. J Immunol. 150(9): 4136-50.

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