General Information
| Product name | Human VEGF165 ELISA Kit |
| Specificity | This assay recognizes both recombinant and natural Human VEGF165. |
| Conjugate | HRP |
| Shipping | This ELISA Kit is shipped at ambient temperature. |
| Storage | Unopened Kit: Store at 2 - 8℃ Opened/Reconstituted Reagents: Please refer to CoA |
| Assay type | Solid Phase Sandwich ELISA (quantitative) |
| Sample type | Serum,Cell supernatant,Plasma |
| Sensitivity | 18.55 pg/mL |
| Assay range | 78.13-5000 pg/mL |
| Materials provided | 1. 96 well microplate coated with Capture Antibody 2. Detection Antibody conjugated to HRP 3. Standards 4. Wash Buffer Concentrate 5. Dilution Buffer Concentrate 6. Color Reagent A 7. Color Reagent B 8. Stop Solution |
| Product overview | This Human VEGF165 ELISA Kit is an enzyme-linked immunosorbent assay for the quantitative measurement of Human VEGF-A protein in Serum,Cell supernatant,Plasma . It contains recombinant Human VEGF-A, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use. |
| Interference | Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human VEGF165 control were assayed for interference. No significant interference was observed. Interference was observed with recombinant human VEGFR1 and Mouse VEGFR1 at concentrations ≥ 0.5 ng/mL, and with recombinant Mouse VEGF164 at concentrations ≥ 5.0 ng/mL. |
Synonyms: L-VEGF ELISA Kit, Human; MVCD1 ELISA Kit, Human; VEGF ELISA Kit, Human; VEGF-A ELISA Kit, Human; VPF ELISA Kit, Human
Image / Experimental Information
| Image 1 Description | This standard curve is only for demonstration purposes. A standard curve should be generated for each assay. |
| Image 1 Alt Text | Human VEGFA ELISA Kit Standard Curve 31479 |
| Image 1 URL | Source image |
| Image 2 Description | This standard curve is only for demonstration purposes. A standard curve should be generated for each assay. |
| Image 2 Alt Text | Human VEGFA ELISA Kit Specificity 31480 |
| Image 2 URL | Source image |
Background Information
| Full Name | vascular endothelial growth factor A |
| Description | Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor. |
| Research Areas | |
| Related Pathways | |
| Tags | - Cancer Immunotherapy
- Immune Checkpoint
- Immunotherapy
- Targeted Therapy
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| References | - Woolard J. et al. (2004) VEGF165b, an inhibitory vascular endothelial growth factor splice variant: mechanism of action, in vivo effect on angiogenesis and endogenous protein expression. Cancer Res. 64(21): 7822-7835.
- Jia SF, et al. (2008) VEGF165 is necessary to the metastatic potential of Fas(-) osteosarcoma cells but will not rescue the Fas(+) cells. J Exp Ther Oncol. 7(2): 89-97.
- Cimpean AM, et al. (2008) Vascular endothelial growth factor A (VEGF A) as individual prognostic factor in invasive breast carcinoma. Rom J Morphol Embryol. 49(3): 303-8.
- Hamdollah Zadeh MA, et al. (2008) VEGF-mediated elevated intracellular calcium and angiogenesis in human microvascular endothelial cells in vitro are inhibited by dominant negative TRPC6. Microcirculation. 15(7): 605-14.
- Eisenach PA, et al. (2010) MT1-MMP regulates VEGF-A expression through a complex with VEGFR-2 and Src. J Cell Sci. 123(Pt 23):4182-4193.
- Claesson-Welsh L (2010) Gremlin: vexing VEGF receptor agonist. Blood. 116(18):3386-7.
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