| Catalog No. | Size | Price (USD) |
| CT031-C08H-1 | 1 mg | $4,366.31 |
| CT031-C08H-20 | 20 µg | $380.64 |
| CT031-C08H-50 | 50 µg | $765.73 |
| CT031-C08H-100 | 100 µg | $669.46 |
General Information
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. ≥ 90 % as determined by SEC-MALS(Routinely tested). |
| Activity | 1.Loaded Cynomolgus FcRn recombinant protein (Cat. CT031-C08H) on His1K Biosensor, can bind IgG1 Fc (Cat. 10702-HNAC) with an affinity constant of 0.032 μM as determined in a BLI assay (Sartorius Octet Red384)(Routinely tested). 2.Captured Cynomolgus FcRn recombinant protein (Cat. CT031-C08H) on Anti-His Chip can bind Anti-VEGF (Research Grade Bevacizumab Biosimilar) (IgG1) with an affinity constant of 0.09 µM as determined in an SPR assay (Biacore T200)(Routinely tested). 3.Captured Cynomolgus FcRn recombinant protein (Cat. CT031-C08H) on Anti-His Chip can bind Anti-PD1(Research grade Nivolumab biosimilar) (IgG4) with an affinity constant of 0.18 µM as determined in an SPR assay (Biacore T200)(Routinely tested). 4.Recombinant Cynomolgus FCGRT & B2M Heterodimer Protein (Cat: CT031-C08H) captured on CM5 chip via Anti-His antibody, can bind anti-ERBB2(Research grade trastuz*mab biosimilar) with an affinity constant of 1.290 μM as determined in an SPR assay (Biacore 8K) (Routinely tested). |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of cynomolgus FCGRT (Q8SPV9) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified. |
| Accession# | Q8SPV9 & Q8SPW0 |
| Expressed Host | HEK293 Cells |
| Species | Cynomolgus |
| Predicted N Terminal | Ala 24 & Ile 21 |
| Molecule Mass | The recombinant heterodimer of cynomolgus FCGRT&B2M comprises 384 (285+99) amino acids and has a calculated molecular mass of 43.5 (31.9+ 11.6) KDa. The apparent molecular mass of cynomolgus FCGRT&B2M heterodimer is approximately 35 & 12 KDa respectively in SDS-PAGE The molecular weight of this protein is around 40-60 kDa verified by SEC-MALS (Routinely tested). |
Image / Experimental Information
| Image 1 Alt Text | Cynomolgus FCGRT & B2M Protein 13004 |
| Image 1 URL | Source image |
| Image 2 Alt Text | Cynomolgus FCGRT & B2M Heterodimer Protein 35445 |
| Image 2 URL | Source image |
| Image 3 Alt Text | Cynomolgus FCGRT & B2M Heterodimer Protein 35464 |
| Image 3 URL | Source image |
| Image 4 Alt Text | Cynomolgus FCGRT & B2M Heterodimer Protein 35465 |
| Image 4 URL | Source image |
| Image 5 Alt Text | Cynomolgus FCGRT & B2M Heterodimer Protein 35500 |
| Image 5 URL | Source image |
Background Information
| Full Name | Fc fragment of IgG, receptor, transporter, alpha |
| Research Areas |
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title: Nucleic acids encoding modified immunoglobulin Fc regions
authors: Geoffrey Hale; Ian Wilkinson
date: 2025-12-16
cited_by_count: 0
techniques: Binding Assay
bioz_score: 94
title: Modified immunoglobulin Fc regions
authors: Geoffrey Hale; Ian Wilkinson
date: 2024-07-16
cited_by_count: 0
techniques: Binding Assay
bioz_score: 94
title: Distribution of FcRn Across Species and Tissues
authors: Sari Latvala; Bjoern Jacobsen; ...; Sven Kronenberg
journal: Journal of Histochemistry & Cytochemistry
date: 2017-06-01
pmid: 28402755
doi: 10.1369/0022155417705095
pmcid: 5625855
cited_by_count: 129
techniques: Derivative Assay; Recombinant; SDS Page; Western Blot
bioz_score: 94
