| Catalog No. | Size | Price (USD) |
| P47-10H-10 | 10 µg | $974.57 |
| P47-10H-50 | 50 µg | $3,062.93 |
| P47-10H-500 | 500 µg | $5,551.19 |
General Information
| Formulation | Supplied as sterile 50 mM sodium phosphate, pH 7.5, 300mM NaCl, 50-200mM imidazole, 0-0.1mM PMSF, 0.2-1.0mM DTT, 10-25% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Shipping | Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
| Stability & Storage | Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Purity | For specific information on a given lot, see related technical data sheet. |
| Activity | For specific information on a given lot, see related technical data sheet. |
| Protein Construction | Recombinant full length human AMPK (combination of A1/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using C-terminal His tags. |
| Expressed Host | Baculovirus-Insect Cells |
| Species | Human |
| Molecule Mass | ~68kDa (A1), ~38kDa (B1), ~40kDa (G1) |
| Technical Data Sheet by Lot # | A4659-11 F5079-11 L5210-5 S3657-2 Y4134-10 |
Image / Experimental Information
| Image 1 Description | Determination of ATP Km for recombinant protein kinases AMPK-A1/B1/G1)-Cat. No.: P47-10H) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 1 Alt Text | Human AMPK (G1/B1/A1) Heterotrimer Protein 48769 |
| Image 1 URL | Source image |
| Image 2 Description | Determination of ATP Km for recombinant protein kinases AMPK-A1/B1/G1)-Cat. No.: P47-10H) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 2 Alt Text | Human AMPK (G1/B1/A1) Heterotrimer Protein 49497 |
| Image 2 URL | Source image |
| Image 3 Description | Determination of ATP Km for recombinant protein kinases AMPK-A1/B1/G1)-Cat. No.: P47-10H) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 3 Alt Text | Human AMPK (G1/B1/A1) Heterotrimer Protein 49498 |
| Image 3 URL | Source image |
title: AMPK senses cellular levels of nicotinamide adenine dinucleotide
authors: Wilson Niall; Rabanal Ruiz Yoana; ...; Korolchuk Viktor I.
journal: bioRxiv
date: 2026-05-21
doi: 10.64898/2026.05.19.726234
cited_by_count: 0
techniques: Activity Assay; Binding Assay; Comparison; Concentration Assay; Expressing; Glo Assay; Immunoprecipitation; In Vitro; Incubation; Kinase Assay; Lysis; Mutagenesis; Recombinant; Residue; Stable Transfection; Titration; Western Blot
bioz_score: 94
title: Salicylate-Elicited Activation of AMP-Activated Protein Kinase Directly Triggers Degradation of C-Myc in Colorectal Cancer Cells.
authors: Ana Laura S A Matos; Ashley J Ovens; ...; José M A Moreira
journal: Cells
date: 2025-02-25
pmid: 39996767
cited_by_count: 1
techniques: Recombinant
bioz_score: 94
title: Targeting the CSF-1/CSF-1R Axis: Exploring the Potential of CSF1R Inhibitors in Neurodegenerative Diseases
authors: Jihyun Baek; Joonhong Jun; ...; Jung-Mi Hah
journal: Journal of Medicinal Chemistry
date: 2024-03-26
pmid: pm38530425
cited_by_count: 0
techniques: Derivative Assay; Real-time Polymerase Chain Reaction; Software
bioz_score: 94
title: Epinephrine inhibits PI3Kα via the Hippo kinases
authors: Ting-Yu Lin; Shakti Ramsamooj; ...; Marcus D. Goncalves
journal: Cell Reports
date: 2024-01-10
pmid: 38060450
doi: 10.1016/j.celrep.2023.113535
pmcid: 10809223
cited_by_count: 1
techniques: Double Knockout; Fluorescence; Isolation; Mutagenesis; Plasmid Preparation; Recombinant; Software
bioz_score: 94
title: Hypoxanthine phosphoribosyl transferase 1 metabolizes temozolomide to activate AMPK for driving chemoresistance of glioblastomas
authors: Jianxing Yin; Xiefeng Wang; ...; Xu Qian
journal: Nature Communications
date: 2023-09-22
pmid: 37737247
doi: 10.1038/s41467-023-41663-2
pmcid: 10516874
cited_by_count: 22
techniques: Activity Assay; Autoradiography; Clone Assay; Comparison; Double Knockout; Expressing; Immunoprecipitation; In Vitro; Incubation; Mutagenesis; Phosphorylation Assay; Purification; SDS Page; Transfection; Western Blot; shRNA
bioz_score: 94
title: Carbamate JNK3 Inhibitors Show Promise as Effective Treatments for Alzheimer's Disease: In Vivo Studies on Mouse Models.
authors: Joonhong Jun; Hyungwoo Moon; ...; Jung-Mi Hah
journal: Journal of Medicinal Chemistry
date: 2023-05-12
pmid: 37094094
doi: 10.1021/acs.jmedchem.3c00393
cited_by_count: 4
techniques: Binding Assay; Derivative Assay
bioz_score: 94
title: Identification of Phosphorylation and Other Post-Translational Modifications in the Central C4C5 Domains of Murine Cardiac Myosin Binding Protein C
authors: Chang Yoon Doh; Katherine L. Dominic; ...; Julian E. Stelzer
journal: ACS Omega
date: 2022-04-18
pmid: 35573219
doi: 10.1021/acsomega.2c00799
pmcid: 9089392
cited_by_count: 3
techniques: Concentration Assay; Molecular Weight; Mutagenesis
bioz_score: 94
title: AMPK leads to phosphorylation of the transcription factor Nrf2, tuning transactivation of selected target genes.
authors: Manuel Matzinger; Katrin Fischhuber; ...; Elke H Heiss
journal: Redox Biology
date: 2021-06-18
pmid: 31805502
doi: 10.1016/j.redox.2019.101393
cited_by_count: 127
techniques: Cell Culture; Recombinant
bioz_score: 94
title: The protein kinase activity of fructokinase A specifies the antioxidant responses of tumor cells by phosphorylating p62
authors: Daqian Xu; Xinjian Li; ...; Zhimin Lu
journal: Science Advances
date: 2019-04-01
pmid: 31032410
doi: 10.1126/sciadv.aav4570
pmcid: 6482012
cited_by_count: 59
techniques: Autoradiography; Cell Culture; Clone Assay; Double Knockout; Expressing; Immunoprecipitation; In Vitro; Incubation; Infection; Kinase Assay; Knock-In; Purification; Transfection; Western Blot; shRNA
bioz_score: 94
title: IPMK Mediates Activation of ULK Signaling and Transcriptional Regulation of Autophagy Linked to Liver Inflammation and Regeneration
authors: Prasun Guha; Richa Tyagi; ...; Solomon H. Snyder
journal: Cell Reports
date: 2019-03-14
pmid: 30840891
doi: 10.1016/j.celrep.2019.02.013
pmcid: 6494083
cited_by_count: 40
techniques: DNA Purification; Generated; In Situ; Plasmid Preparation; Purification; Recombinant; SYBR Green Assay; Software; Staining; shRNA
bioz_score: 94
title: AMPK‐mediated degradation of Nav1.5 through autophagy
authors: Xuehua Liu; Zheng Chen; ...; Wei Xu
journal: The FASEB Journal
date: 2019-02-13
doi: 10.1096/fj.201801583rr
cited_by_count: 13
techniques: Binding Assay; Cell Culture; Construct; Expressing; Immunoprecipitation; In Vitro; Incubation; Kinase Assay; Mutagenesis; Phospho-proteomics; Pull Down Assay; Residue; Staining; Transfection; Western Blot
bioz_score: 94
title: The PLAG1-GDH1 axis promotes anoikis resistance and tumor metastasis through CamKK2-AMPK signaling in LKB1-deficient lung cancer
authors: Lingtao Jin; Jaemoo Chun; ...; Sumin Kang
journal: Molecular cell
date: 2019-01-04
pmid: 29249655
doi: 10.1016/j.molcel.2017.11.025
pmcid: 5777230
cited_by_count: 211
techniques: ATP Bioluminescent Assay; Activation Assay; Caspase-Glo Assay; Chromatin Immunoprecipitation; Expressing; Isolation; Malate Assay; Plasmid Preparation; Recombinant; Sequencing; Software; shRNA
bioz_score: 94
title: Conversion of PRPS hexamer to monomer by AMPK-mediated phosphorylation inhibits nucleotide synthesis in response to energy stress
authors: Xu Qian; Xinjian Li; ...; Zhimin Lu
journal: Cancer Discovery
date: 2019-01-01
pmid: 29074724
doi: 10.1158/2159-8290.CD-17-0712
pmcid: 5760453
cited_by_count: 50
techniques: Cell Culture; FLAG-tag; Transfection
bioz_score: 94
title: 2-[2-(4-(trifluoromethyl)phenylamino)thiazol-4-yl]acetic acid (Activator-3) is a potent activator of AMPK
authors: Navneet Bung; Sobhitha Surepalli; ...; Parimal Misra
journal: Scientific Reports
date: 2018-06-25
pmid: 29942003
doi: 10.1038/s41598-018-27974-1
pmcid: 6018554
cited_by_count: 10
techniques: Activation Assay; Activity Assay; Binding Assay; Concentration Assay; Construct; De-Phosphorylation Assay; In Vitro; In-Cell ELISA; Incubation; Kinase Assay; Over Expression; Purification; Recombinant
bioz_score: 94
title: Nucleus-translocated ACSS2 promotes the gene transcription for lysosomal biogenesis and autophagy
authors: Xinjian Li; Willie Yu; ...; Zhimin Lu
journal: Molecular cell
date: 2018-06-01
pmid: 28552616
doi: 10.1016/j.molcel.2017.04.026
pmcid: 5521213
cited_by_count: 222
techniques: Chromatin Immunoprecipitation; Double Knockout; Fractionation; Plasmid Preparation; Real-time Polymerase Chain Reaction; Recombinant; SYBR Green Assay; Sequencing; Software; Transduction; Transfection; shRNA
bioz_score: 94
title: A Functional Signature Ontology (FUSION) screen detects an AMPK inhibitor with selective toxicity toward human colon tumor cells
authors: Binita Das; Beth K. Neilsen; ...; Robert E. Lewis
journal: Scientific Reports
date: 2018-02-28
pmid: 29491475
doi: 10.1038/s41598-018-22090-6
pmcid: 5830883
cited_by_count: 13
techniques: Activity Assay; Inhibition; Recombinant; Western Blot
bioz_score: 94
title: Phosphorylation of Cytochrome c Threonine 28 Regulates Electron Transport Chain Activity in Kidney
authors: Gargi Mahapatra; Ashwathy Varughese; ...; Maik Hüttemann
journal: The Journal of Biological Chemistry
date: 2017-01-06
pmid: 27758862
doi: 10.1074/jbc.M116.744664
pmcid: 5217700
cited_by_count: 39
techniques: Control; In Vitro; Incubation; Mass Spectrometry; Phospho-proteomics; Protease Inhibitor; Solvent; Western Blot
bioz_score: 94
title: Direct Binding of Arsenic Trioxide to AMPK and Generation of Inhibitory Effects on Acute Myeloid Leukemia Precursors
authors: Elspeth M. Beauchamp; Ewa M. Kosciuczuk; ...; Leonidas C. Platanias
journal: Molecular Cancer Therapeutics
date: 2014-10-24
doi: 10.1158/1535-7163.mct-14-0665-t
cited_by_count: 23
techniques: Concentration Assay; Incubation; Isolation; Lysis; Recombinant; Western Blot
bioz_score: 93
title: Identification of a novel phosphorylation site on TBC1D4 regulated by AMP-activated protein kinase in skeletal muscle
authors: Jonas T. Treebak; Eric B. Taylor; ...; Laurie J. Goodyear
journal: American Journal of Physiology - Cell Physiology
date: 2011-02-01
pmid: 19923418
doi: 10.1152/ajpcell.00297.2009
pmcid: 2822490
cited_by_count: 85
techniques: Activity Assay; Chromatography; Electrotransfer; FLAG-tag; Immunoprecipitation; In Vitro; Incubation; Mass Spectrometry; Positive Control; Recombinant; SDS Page; Western Blot
bioz_score: 94
title: Discovery of TBC1D1 as an Insulin-, AICAR-, and Contraction-stimulated Signaling Nexus in Mouse Skeletal Muscle
authors: Eric B. Taylor; Ding An; ...; Laurie J. Goodyear
journal: Journal of Biological Chemistry
date: 2008-04-01
doi: 10.1074/jbc.m708839200
cited_by_count: 198
techniques: Activation Assay; Amplification; Control; Immunoprecipitation; In Vitro; In Vivo; Incubation; Molecular Weight; Phospho-proteomics; Recombinant; Suspension; Western Blot
bioz_score: 94
MDSD
