| Catalog No. | Size | Price (USD) |
| A03-18H-10 | 10 µg | $515.43 |
| A03-18H-50 | 50 µg | $1,729.93 |
| A03-18H-500 | 500 µg | $3,907.16 |
General Information
| Formulation | Supplied as sterile 50 mM sodium phosphate, pH 7.5, 300mM NaCl, 50-200mM imidazole, 0-0.1mM PMSF, 0.2-1.0mM DTT, 10-25% glycerol Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Shipping | Enzymes are highly recommended to be shipped at frozen temperature with dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
| Stability & Storage | Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |
| Purity | For specific information on a given lot, see related technical data sheet. |
| Activity | For specific information on a given lot, see related technical data sheet. |
| Protein Construction | Recombinant human ABL1 (27-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. |
| Expressed Host | Baculovirus-Insect Cells |
| Species | Human |
| Molecule Mass | ~135 kDa |
| Technical Data Sheet by Lot # | E4163-11 J5244-24 O3937-8 U4803-6 U4803-7 V2384-8 Y4267-10 |
Synonyms: ABL Protein, Human; BCR-ABL Protein, Human; bcr/abl Protein, Human; c-ABL Protein, Human; c-ABL1 Protein, Human; CHDSKM Protein, Human; JTK7 Protein, Human; p150 Protein, Human; v-abl Protein, Human
Image / Experimental Information
| Image 1 Description | Determination of ATP Km for recombinant protein kinases ABL1 (Cat. No.: A03-18H) using ADP-GloTM Assay. With saturating substrate concentration, the relative luminescence values were plotted against various ATP concentrations. The Michaelis-Menten equation was then used to generate the Km values. (Validation Experiment) |
| Image 1 Alt Text | Human c-Abl/ABL1 Protein 46289 |
| Image 1 URL | Source image |
Background Information
| Full Name | ABL proto-oncogene 1, non-receptor tyrosine kinase |
| Description | c-Abl belongs to the class of tyrosine kinases and is the prototype of a subfamily which includes two members, c-Abl and Arg (Abl-related gene). Both proteins are localized at the cell membrane, actin cytoskeleton and cytosol, and c-Abl is present in the nucleus as well. c-Abl is a non-receptor tyrosine kinase that participates in multiple signaling pathways linking the cell surface, cytoskeleton, and the nucleus. Recent in vitro studies have also linked c-Abl to amyloid-beta-induced toxicity and tau phosphorylation. c-Abl has been implicated in many cellular processes including differentiation, division, adhesion, death, and stress response. c-Abl is a latent tyrosine kinase that becomes activated in response to numerous extra- and intra-cellular stimuli. The c-Abl protein is a ubiquitously expressed nonreceptor tyrosine kinase involved in the development and function of many mammalian organ systems, including the immune system and bone. It regulates the cellular response to TAM through functional interaction with the estrogen receptor, which suggests c-Abl as a therapeutic target and a prognostic tumor marker for breast cancer. c-Abl also plays a key role in signaling chemokine-induced T-cell migration. In addition, c-Abl contains NLSs (nuclear localization signals) and DNA-binding sequences important for nuclear functions. c-Abl has become an important therapeutic target in human chronic myeloid leukaemia. |
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| References |
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title: Imaging the Binding Between Dasatinib and Its Target Protein in Living Cells Using an SLP Tag System on Intracellular Compartments.
authors: Da Kyeong Park; Sang-Hee Lee; ...; Kyung-Bok Lee
journal: International Journal of Molecular Sciences
date: 2025-06-26
pmid: 40565169
cited_by_count: 0
techniques: In Vitro; Kinase Assay; Recombinant
bioz_score: 93
title: Epinephrine inhibits PI3Kα via the Hippo kinases
authors: Ting-Yu Lin; Shakti Ramsamooj; ...; Marcus D. Goncalves
journal: Cell Reports
date: 2024-01-10
pmid: 38060450
doi: 10.1016/j.celrep.2023.113535
pmcid: 10809223
cited_by_count: 1
techniques: Double Knockout; Fluorescence; Isolation; Mutagenesis; Plasmid Preparation; Recombinant; Software
bioz_score: 92
title: 2-amino-3-carbonyl imidazopyridine and pyrazolopyridine compounds
authors: Jinhwa Lee; Suyeon Jo; ...; Inyoung Yang
date: 2023-09-05
cited_by_count: 0
techniques: Activity Assay; Concentration Assay; Incubation; Inhibition; Kinase Assay; Recombinant; Software
bioz_score: 93
title: Imidazopyridinyl compounds and use thereof for treatment of neurodegenerative disorders
authors: Jinhwa Lee; Suyeon Jo; ...; Seung Mook Lim
date: 2021-08-24
cited_by_count: 0
techniques: Activity Assay; Incubation; Kinase Assay; Recombinant
bioz_score: 93
title: Benzothiazol compounds and methods using the same for treating neurodegenerative disorders
authors: Jinhwa Lee; Suyeon Jo; ...; Mingzhu Fan
date: 2021-04-06
cited_by_count: 0
techniques: Activity Assay; Incubation; Kinase Assay; Recombinant
bioz_score: 93
title: Mapping phospho-catalytic dependencies of therapy-resistant tumours reveals actionable vulnerabilities
authors: Jean-Philippe Coppé; Miki Mori; ...; Laura J. van ‘t Veer
journal: Nature cell biology
date: 2020-04-20
pmid: 31160710
doi: 10.1038/s41556-019-0328-z
pmcid: 7170003
cited_by_count: 30
techniques: Activity Assay; Cell Culture; Concentration Assay; Control; Functional Assay; Positive Control; Purification; Recombinant
bioz_score: 92
title: c-Abl phosphorylation of Yin Yang 1's conserved tyrosine 254 in the spacer region modulates its transcriptional activity.
authors: Susan I Daraiseh; Ari Kassardjian; ...; Myra M Hurt
journal: Biochimica et biophysica acta. Molecular cell research
date: 2018-12-11
pmid: 29807053
doi: 10.1016/j.bbamcr.2018.05.014
cited_by_count: 5
techniques: Dot Blot; In Vitro; In Vivo; Incubation; Kinase Assay; Purification
bioz_score: 93
MDSD
