| Catalog No. | Size | Price (USD) |
| 10692-H27H-B-20 | 20 µg | $441.37 |
| 10692-H27H-B-100 | 100 µg | $1,019.00 |
General Information
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Biotin/Protein Ratio | 0.5-1 as determined by the HABA assay. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | ≥ 95 % as determined by SDS-PAGE. ≥90 % as determined by SEC-HPLC. |
| Activity | Immobilized Recombinant Human HGF / Hepatocyte Growth Factor Protein Cat:10463-HNAS) at 2μg/mL (100μL/well) can bind Recombinant Human c-Met / HGFR Protein (His & AVI Tag), Biotinylated (Cat:10692-H27H-B), the EC 50 is 10-30 ng/mL. |
| Protein Construction | A DNA sequence encoding the human MET (NP_000236.2) (Met1-Thr932) was expressed with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
| Accession# | NP_000236.2 |
| Expressed Host | HEK293 Cells |
| Species | Human |
| Predicted N Terminal | Glu 25 |
| Molecule Mass | The recombinant human MET consists of 934 amino acids and predicts a molecular mass of 104.9 kDa. |
Synonyms: AUTS9 Protein, Human; c-Met Protein, Human; DA11 Protein, Human; DFNB97 Protein, Human; HGFR Protein, Human; RCCP2 Protein, Human
Image / Experimental Information
| Image 1 Alt Text | Human c-MET Protein 35761 |
| Image 1 URL | Source image |
| Image 2 Alt Text | Human c-MET Protein 36294 |
| Image 2 URL | Source image |
| Image 3 Alt Text | Human c-MET Protein 27711 |
| Image 3 URL | Source image |
Background Information
| Full Name | MET proto-oncogene, receptor tyrosine kinase |
| Description | Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that is overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair, or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer. |
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| References |
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title: Responsive DNA artificial cells for contact and behavior regulation of mammalian cells.
authors: Miao Wang; Hexin Nan; ...; Zhou Nie
journal: Nature Communications
date: 2025-03-12
pmid: 40069211
doi: 10.1038/s41467-025-57770-1
cited_by_count: 7
techniques: Incubation; Labeling; Recombinant
bioz_score: 95
title: Reduction of therapeutic antibody self-association using yeast-display selections and machine learning
authors: Emily K. Makowski; Hongwei Chen; ...; Peter M. Tessier
journal: mAbs
date: 2022-11-26
pmid: 36433737
pmcid: 9704398
cited_by_count: 20
techniques: Binding Assay; FACS; Flow Cytometry; Fluorescence; Incubation; Mutagenesis; Sequencing
bioz_score: 95
title: Co-optimization of therapeutic antibody affinity and specificity using machine learning models that generalize to novel mutational space.
authors: Emily K Makowski; Patrick C Kinnunen; ...; Peter M Tessier
journal: Nature Communications
date: 2022-07-07
pmid: 35778381
cited_by_count: 74
techniques: Binding Assay; Incubation
bioz_score: 95
title: A Highly Potent and Specific MET Therapeutic Protein Antagonist with Both Ligand-Dependent and Ligand-Independent Activity
authors: Shane A. Olwill; Christian Joffroy; ...; Laurent Audoly
journal: Molecular Cancer Therapeutics
date: 2013-09-03
doi: 10.1158/1535-7163.mct-13-0318
cited_by_count: 27
techniques: Binding Assay; Control
bioz_score: 93
