| Catalog No. | Size | Price (USD) |
| 10774-H02H-1 | 1 mg | $2,479.38 |
| 10774-H02H-50 | 50 µg | $342.14 |
| 10774-H02H-100 | 100 µg | $476.92 |
General Information
| Formulation | Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Shipping | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
| Stability & Storage | Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution | To find reconstitution info, click on 'COA' at the top of the page, enter the lot number and product size for the lot-specific COA. |
| Purity | > 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC. |
| Activity | 1.Immobilized Recombinant Human CD40L / CD154 / TNFSF5 Protein (His Tag) (Cat: 10239-H08E) at 2 μg/mL (100 μL/well) can bind Recombinant Human CD40 / TNFRSF5 Protein (ECD, Fc Tag) (Cat: 10774-H02H), the EC 50 is 10-30 ng/mL. 2.Loaded Recombinant Human CD40/TNFRSF5 Protein, hFc Tag (Cat. No. 10774-H02H) can bind Recombinant Human CD40 Ligand/TNFSF5 Protein, His Tag (Cat. No. 10239-H08E) with an affinity constant of 3.06 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). |
| Protein Construction | A DNA sequence encoding the human CD40 (NP_001241.1) (Met1-Arg193) was expressed with the Fc region of human IgG1 at the C-terminus. |
| Accession# | NP_001241.1 |
| Expressed Host | HEK293 Cells |
| Species | Human |
| Predicted N Terminal | Glu 21 |
| Molecule Mass | The recombinant human CD40 consists 414 amino acids and predicts a molecular mass of 46.2 kDa. As a result of glycosylation, the apparent molecular mass of human CD40 is approximately 54.1 kDa in SDS-PAGE under reducing conditions. |
Synonyms: Bp50 Protein, Human; CDW40 Protein, Human; p50 Protein, Human; TNFRSF5 Protein, Human
Image / Experimental Information
| Image 1 Alt Text | Human CD40/TNFRSF5 Protein 9105 |
| Image 1 URL | Source image |
| Image 2 Alt Text | Human CD40 Protein 34338 |
| Image 2 URL | Source image |
| Image 3 Alt Text | Human CD40 Protein 28000 |
| Image 3 URL | Source image |
| Image 4 Alt Text | Human CD40/TNFRSF5 Protein 42034 |
| Image 4 URL | Source image |
| Image 5 Alt Text | Human CD40 Ligand/TNFSF5 Protein 40963 |
| Image 5 URL | Source image |
Background Information
| Full Name | CD40 molecule, TNF receptor superfamily member 5 |
| Description | CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. |
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| References |
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title: Improved safety of chimeric antigen receptor T cells indirectly targeting antigens via switchable adapters
authors: Hyung Bae Park; Ki Hyun Kim; ...; Kyungho Choi
journal: Nature Communications
date: 2024-11-18
pmid: 39557825
doi: 10.1038/s41467-024-53996-7
pmcid: 11574259
cited_by_count: 10
techniques: Binding Assay; Cell Counting; Cell Culture; Clone Assay; Co-Culture Assay; Comparison; Construct; Control; Enzyme-linked Immunosorbent Assay; Expressing; Flow Cytometry; Fluorescence; Imaging; In Vivo; Injection; Irradiation; Isolation; Knock-Out; Labeling; Luciferase; Neutralization; Recombinant; Staining; Synthesized; Transduction; Two Tailed Test
bioz_score: 93
title: Antibody binding specifically to CD40 and use thereof
authors: Gil Yong Ji; Kwon Pyo Hong; ...; Sangsoon Yoon
date: 2024-02-20
cited_by_count: 0
techniques: Blocking Assay; Concentration Assay; Filtration; Incubation; Recombinant; Saline
bioz_score: 94
title: DuoBody-CD40x4-1BB induces dendritic-cell maturation and enhances T-cell activation through conditional CD40 and 4-1BB agonist activity
authors: Alexander Muik; Homer C Adams III; ...; Maria Jure-Kunkel
journal: Journal for Immunotherapy of Cancer
date: 2022-06-01
pmid: 35688554
doi: 10.1136/jitc-2021-004322
pmcid: 9189854
cited_by_count: 25
techniques: Recombinant; Software
bioz_score: 93
title: Bispecific antibodies increase the therapeutic window of CD40 agonists through selective dendritic cell targeting.
authors: Ran Salomon; Hagar Rotem; ...; Rony Dahan
journal: Nature cancer
date: 2022-04-19
pmid: 35190724
doi: 10.1038/s43018-022-00329-6
cited_by_count: 47
techniques: Binding Assay; Blocking Assay; Enzyme-linked Immunosorbent Assay; Fast Protein Liquid Chromatography; Incubation; Recombinant; Size-exclusion Chromatography
bioz_score: 93
title: Anti-CD40 antibody KPL-404 inhibits T cell-mediated activation of B cells from healthy donors and autoimmune patients
authors: John Marken; Sujatha Muralidharan; Natalia V. Giltiay
journal: Arthritis research & therapy
date: 2021-01-06
pmid: 33407802
doi: 10.1186/s13075-020-02372-z
pmcid: 7789801
cited_by_count: 11
techniques: Activation Assay; Binding Assay; Cell Culture; Expressing; Flow Cytometry; Imaging; Incubation; Labeling; Recombinant; Staining; Standard Deviation; Transformation Assay
bioz_score: 93
title: CD40 binding antibodies and uses thereof
authors: Wenqi Hu; Jiangmei Li; Feng Li
date: 2020-05-19
cited_by_count: 0
techniques: Adjuvant; Enzyme-linked Immunosorbent Assay; Injection; Recombinant; Sonication
bioz_score: 94
title: Antibodies specific to CD40 and uses thereof
authors: Wenqi Hu; Jiangmei Li; Feng Li
date: 2020-05-19
cited_by_count: 0
techniques: Adjuvant; Enzyme-linked Immunosorbent Assay; Incubation; Injection; Recombinant; Sonication
bioz_score: 94
title: CD40 specific antibodies and uses thereof
authors: Wenqi Hu; Jiangmei Li; Feng Li
date: 2020-05-19
cited_by_count: 0
techniques: Adjuvant; Binding Assay; Blocking Assay; Enzyme-linked Immunosorbent Assay; Incubation; Injection; Recombinant; Sonication
bioz_score: 93
title: Anti-CD40 antibodies and uses thereof
authors: Wenqi Hu; Jiangmei Li; Feng Li
date: 2020-05-12
cited_by_count: 0
techniques: Adjuvant; Binding Assay; Blocking Assay; Enzyme-linked Immunosorbent Assay; Incubation; Injection; Recombinant; Sonication
bioz_score: 93
title: Antibodies binding CD40 and uses thereof
authors: Wenqi Hu; Jiangmei Li; Feng Li
date: 2020-02-25
cited_by_count: 0
techniques: Adjuvant; Binding Assay; Blocking Assay; Enzyme-linked Immunosorbent Assay; Incubation; Injection; Recombinant; Sonication
bioz_score: 94
